Anti-TCF4/12 Antibody | MyBioSource MBS8218874 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-TCF4/12 Antibody

catalog :

MBS8218874

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of TCF4/12 expression in K562 (A), A549 (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of TCF4/12 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

product information

catalog number :

MBS8218874

products type :

Antibody

products full name :

Anti-TCF4/12 Antibody

products short name :

[TCF4/12]

products name syn :

[TCF4; BHLHB19; ITF2; SEF2; Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2; TCF12; BHLHB20; HEB; HTF4; Transcription factor 12; TCF-12; Class B basic helix-loop-helix protein 20; bHLHb20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4]

other names :

[transcription factor 4 isoform a; Transcription factor 4; transcription factor 4; transcription factor 4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2]

products gene name :

[TCF4]

products gene name syn :

[TCF12]

other gene names :

[TCF4; TCF4; E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19; BHLHB19; ITF2; SEF2; TCF-4; bHLHb19; ITF-2; SEF-2]

uniprot entry name :

ITF2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Chicken, Porcine

sequence length :

671

specificity :

Recognizes endogenous levels of TCF4/12 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipping: Blue ice. Upon delivery aliquot and store at -20° C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB (1/500 - 1/1000). IH (1/100 - 1/200)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TCF4/12. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to TCF4/12

ncbi gi num :

145312267

ncbi acc num :

NP_001077431.1

ncbi gb acc num :

NM_001083962.1

uniprot acc num :

P15884

ncbi pathways :

CDO In Myogenesis Pathway (1270336); Coregulation Of Androgen Receptor Activity Pathway (138085); Corticotropin-releasing Hormone Pathway (920957); Developmental Biology Pathway (1270302); Myogenesis Pathway (1270335); Neural Crest Differentiation Pathway (672460); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Wnt Signaling Pathway NetPath (198799)

ncbi summary :

This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Aug 2011]

uniprot summary :

TCF4: Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5 -CANNTG-3 ). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5 -ACANNTGT-3 or 5 -CCANNTGG-3 . Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2. Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 18q21.1. Cellular Component: transcription factor complex; nucleus. Molecular Function: protein C-terminus binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor activity. Biological Process: transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; protein-DNA complex assembly. Disease: Corneal Dystrophy, Fuchs Endothelial, 3; Pitt-hopkins Syndrome

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage