Anti-Paxillin Antibody | MyBioSource MBS8218302 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Paxillin Antibody

catalog :

MBS8218302

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Paxillin expression in HeLa (A) whole cell lysates.

image 2 :

Immunohistochemical analysis of Paxillin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Paxillin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8218302

products type :

Antibody

products full name :

Anti-Paxillin Antibody

products short name :

[Paxillin]

products name syn :

[Paxillin]

other names :

[paxillin isoform 2; Paxillin; paxillin; paxillin]

products gene name :

[PXN]

other gene names :

[PXN; PXN]

uniprot entry name :

PAXI_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Porcine

sequence length :

591

specificity :

Recognizes endogenous levels of Paxillin protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Paxillin. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Paxillin

ncbi gi num :

170932514

ncbi acc num :

NP_001074324.1

ncbi gb acc num :

NM_001080855.2

uniprot acc num :

P49023

ncbi mol weight :

46,604 Da

ncbi pathways :

Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arf6 Signaling Events Pathway (138034); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); CXCR4-mediated Signaling Events Pathway (137910); Cell Junction Organization Pathway (1270232); Cell-Cell Communication Pathway (1270231); Cell-extracellular Matrix Interactions Pathway (1270237); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864)

ncbi summary :

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

uniprot summary :

PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 12q24.31. Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; stress fiber; plasma membrane; cell cortex; cytosol. Molecular Function: integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding. Biological Process: lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; focal adhesion formation; integrin-mediated signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPK activity; signal complex assembly; cytoskeleton organization and biogenesis; signal transduction; regulation of cell shape; branching morphogenesis of a tube; muscle contraction; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; cell adhesion

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage