Anti-Collagen 4 alpha 5 Antibody | MyBioSource MBS8218169 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Collagen 4 alpha 5 Antibody

catalog :

MBS8218169

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Collagen 4 alpha 5 expression in HeLa (A), mouse brain (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of Collagen 4 alpha 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Collagen 4 alpha 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8218169

products type :

Antibody

products full name :

Anti-Collagen 4 alpha 5 Antibody

products short name :

[Collagen 4 alpha 5]

products name syn :

[Collagen alpha-5(IV) chain]

other names :

[collagen alpha-5(IV) chain isoform 1; Collagen alpha-5(IV) chain; collagen alpha-5(IV) chain; collagen, type IV, alpha 5]

products gene name :

[COL4A5]

other gene names :

[COL4A5; COL4A5; ATS; ASLN; CA54]

uniprot entry name :

CO4A5_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

1685

specificity :

Recognizes endogenous levels of Collagen 4 alpha 5 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

WB, IH, IF/IC

app notes :

WB (1/500 - 1/1000). IHC (1/100 - 1/200). IF/IC(1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 4 alpha 5. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Collagen 4 alpha 5

ncbi gi num :

4502955

ncbi acc num :

NP_000486.1

ncbi gb acc num :

NM_000495.4

uniprot acc num :

P29400

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1270247); Axon Guidance Pathway (1270303); Collagen Biosynthesis And Modifying Enzymes Pathway (1270246); Collagen Formation Pathway (1270245); Developmental Biology Pathway (1270302); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1270244)

ncbi summary :

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

uniprot summary :

COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: Xq22. Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina; neuromuscular junction. Molecular Function: extracellular matrix structural constituent. Biological Process: collagen catabolic process; extracellular matrix disassembly; axon guidance; extracellular matrix organization and biogenesis; neuromuscular junction development. Disease: Alport Syndrome, X-linked

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage