Anti-EIF2S1 (pS51) Antibody | MyBioSource MBS821692 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-EIF2S1 (pS51) Antibody

catalog :

MBS821692

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, cow, zebrafish

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of EIF2S1 (pS51) expression in HeLa (A), PC12 (B), A431 (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of EIF2S1 (pS51) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

product information

catalog number :

MBS821692

products type :

Antibody

products full name :

Anti-EIF2S1 (pS51) Antibody

products short name :

[EIF2S1 (pS51)]

products name syn :

[EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha]

other names :

[eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha]

products gene name :

[EIF2S1]

other gene names :

[EIF2S1; EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha]

uniprot entry name :

IF2A_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Chicken, Pig, Zebrafish

sequence length :

315

specificity :

Recognizes endogenous levels of EIF2S1 (pS51) protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF2S1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to EIF2S1 (pS51)

ncbi gi num :

4758256

ncbi acc num :

NP_004085.1

ncbi gb acc num :

NM_004094.4

uniprot acc num :

P05198

ncbi pathways :

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); BDNF Signaling Pathway (712093); Cap-dependent Translation Initiation Pathway (105967); Eukaryotic Translation Initiation Pathway (105966); Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway (105969); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973); Gene Expression Pathway (105937); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873)

ncbi summary :

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

uniprot summary :

eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections. Protein type: Translation initiation; RNA-binding; Translation. Chromosomal Location of Human Ortholog: 14q23.3. Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; cytosol; nucleus; eukaryotic translation initiation factor 2 complex. Molecular Function: protein binding; translation initiation factor activity; ribosome binding. Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage