Anti-Kalirin Antibody | MyBioSource MBS821543 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Kalirin Antibody

catalog :

MBS821543

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Kalirin expression in HEK293T (A), NIH3T3 (B), H9C2 (C), mouse brain (D) whole cell lysates.

image 2 :

Immunofluorescent analysis of Kalirin staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS821543

products type :

Antibody

products full name :

Anti-Kalirin Antibody

products short name :

[Kalirin]

products name syn :

[DUET; DUO; HAPIP; TRAD; Kalirin; Huntingtin-associated protein-interacting protein; Protein Duo; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain]

other names :

[kalirin isoform 1; Kalirin; kalirin; huntingtin-associated protein interacting protein (duo); serine/threonine kinase with Dbl- and pleckstrin homology domains; serine/threonine-protein kinase with Dbl- and pleckstrin homology domain; kalirin, RhoGEF kinase; Huntingtin-associated protein-interacting protein; Protein Duo; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain]

products gene name :

[KALRN]

other gene names :

[KALRN; KALRN; DUO; CHD5; DUET; TRAD; CHDS5; HAPIP; ARHGEF24; DUET; DUO; HAPIP; TRAD]

uniprot entry name :

KALRN_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat, Monkey

sequence length :

2986

specificity :

Recognizes endogenous levels of Kalirin protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)

app notes :

WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info2 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kalirin. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Kalirin

ncbi gi num :

148839466

ncbi acc num :

NP_001019831.2

ncbi gb acc num :

NM_001024660.3

uniprot acc num :

O60229

ncbi pathways :

Arf6 Downstream Pathway (138038); Axon Guidance Pathway (105688); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1016174); EPHB Forward Signaling Pathway (138047); EPHB-mediated Forward Signaling Pathway (1016176); G Alpha (12/13) Signalling Events Pathway (106041); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548)

ncbi summary :

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

kalirin: a bifunctional enzyme with protein kinase activity and guanine-exchange factor (GEF) activity. A protein kinase of the CAMK group. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity. Genetic variants are associated with susceptibility to coronary heart disease type 5 (CHDS5), a multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Interacts with the C-terminal of peptidylglycine alpha-amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1) Interacts with FASLG. The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity. 6 isoforms of the human protein are produced by alternative splicing. Isoform 2 is brain specific and is highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle. Protein type: Kinase, protein; GEFs, Rac/Rho; Protein kinase, CAMK; GEFs; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CAMK group; Trio family. Chromosomal Location of Human Ortholog: 3q21.2. Cellular Component: cytosol; actin cytoskeleton. Molecular Function: protein serine/threonine kinase activity; Rho guanyl-nucleotide exchange factor activity; protein binding; metal ion binding; guanyl-nucleotide exchange factor activity; ATP binding. Biological Process: vesicle-mediated transport; axon guidance; nervous system development; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; ephrin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of GTPase activity. Disease: Coronary Heart Disease, Susceptibility To, 5

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage