gene knockdown

ARHGDIA siRNA (Mouse)

MBS8214375

15 nmol

365 USD

siRNA

ARHGDIA siRNA (Mouse)

[ARHGDIA]

[C87222; GDI1; Rho GDP-dissociation inhibitor 1; Rho GDI 1; GDI-1; Rho-GDI alpha]

[rho GDP-dissociation inhibitor 1; Rho GDP-dissociation inhibitor 1; rho GDP-dissociation inhibitor 1; Rho GDP dissociation inhibitor (GDI) alpha; GDI-1; Rho-GDI alpha]

[ARHGDIA]

[Arhgdia; Arhgdia; Gdi-1; RhoDGI; Rho-GDI; RhoGDI-1; 5330430M07Rik; C87222; Gdi1; Rho GDI 1]

GDIR1_MOUSE

Mouse

204

ARHGDIA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

> 97%

Lyophilized powder

Shipped at 4 degree C. Store at -20 degree C for one year.

RNA Interference (RNAi)

Source: Synthetic. Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ARHGDIA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

siRNA to inhibit ARHGDIA expression using RNA interference

31982030

NP_598557.3

NM_133796.7

Q99PT1

23,407 Da

Axonal Growth Inhibition (RHOA Activation) Pathway (1254082); Axonal Growth Stimulation Pathway (1254081); Neurotrophin Signaling Pathway (101156); Neurotrophin Signaling Pathway (100064); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428); Rho GTPase Cycle Pathway (1254135); Signal Transduction Pathway (1253992); Signaling By Rho GTPases Pathway (1254134); Signalling By NGF Pathway (1254067); Vasopressin-regulated Water Reabsorption Pathway (143701)

RhoGDI alpha: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Monomer. Forms a heterodimer with RAC1. Interacts with FER. Belongs to the Rho GDI family. Protein type: Cell adhesion; Motility/polarity/chemotaxis; G protein regulator, misc.; Apoptosis; Cell development/differentiation. Cellular Component: extracellular space; membrane; cytoplasm; immunological synapse; intracellular; cytosol. Molecular Function: protein binding; Rho GTPase binding; Rho GDP-dissociation inhibitor activity; Hsp90 protein binding; protein kinase binding; fatty acid binding; GTPase activator activity. Biological Process: regulation of cell shape; regulation of protein localization; regulation of catalytic activity; negative regulation of cell migration; Rho protein signal transduction

15 nmol

365 USD

30 nmol

515