Anti-MDA5 Antibody | MyBioSource MBS821417 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-MDA5 Antibody

catalog :

MBS821417

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of MDA5 expression in HepG2 (A), THP1 (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of MDA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of MDA5 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS821417

products type :

Antibody

products full name :

Anti-MDA5 Antibody

products short name :

[MDA5]

products name syn :

[MDA5; RH116; Interferon-induced helicase C domain-containing protein 1; Clinically amyopathic dermatomyositis autoantigen 140 kDa; CADM-140 autoantigen; Helicase with 2 CARD domains; Helicard; Interferon-induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116]

other names :

[interferon-induced helicase C domain-containing protein 1; Interferon-induced helicase C domain-containing protein 1; interferon-induced helicase C domain-containing protein 1; helicard; CADM-140 autoantigen; RIG-I-like receptor 2; helicase with 2 CARD domains; RNA helicase-DEAD box protein 116; murabutide down-regulated protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; melanoma differentiation associated protein-5; melanoma differentiation-associated protein 5; clinically amyopathic dermatomyositis autoantigen 140 kDa; interferon induced with helicase C domain 1; Clinically amyopathic dermatomyositis autoantigen 140 kDa; CADM-140 autoantigen; Helicase with 2 CARD domains; Helicard; Interferon-induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116]

products gene name :

[IFIH1]

other gene names :

[IFIH1; IFIH1; AGS7; Hlcd; MDA5; MDA-5; RLR-2; IDDM19; MDA5; RH116; CADM-140 autoantigen; Helicard; MDA-5; RLR-2]

uniprot entry name :

IFIH1_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Rat

sequence length :

1025

specificity :

Recognizes endogenous levels of MDA5 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MDA5. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to MDA5

ncbi gi num :

27886568

ncbi acc num :

NP_071451.2

ncbi gb acc num :

NM_022168.3

uniprot acc num :

Q9BYX4

ncbi mol weight :

25,129 Da

ncbi pathways :

Hepatitis B Pathway (694606); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Immune System Pathway (106386); Influenza A Pathway (217173); Influenza A Pathway (217150); Innate Immune System Pathway (106387); Measles Pathway (213306); Measles Pathway (213277); NF-kB Activation Through FADD/RIP-1 Pathway Mediated By Caspase-8 And -10 (187099)

ncbi summary :

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]

uniprot summary :

IFIH1: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2 -O- methylation at their 5 cap and long-dsRNA ( 1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. Monomer in the absence of ligands and homodimerizes in the presence of dsRNA ligands. Can assemble into helical or linear polymeric filaments on long dsRNA. Interacts with MAVS/IPS1. Interacts with V protein of Simian virus 5, Human parainfluenza virus 2, Mumps virus, Sendai virus and Hendra virus. Binding to paramyxoviruses V proteins prevents IFN-beta induction, and the further establishment of an antiviral state. Interacts with PCBP2. Interacts with NLRC5. Interacts with PIAS2-beta. Interacts with DDX60. By interferon (IFN) and TNF. Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Helicase; EC 3.6.4.13. Chromosomal Location of Human Ortholog: 2q24. Cellular Component: cytosol; nucleus. Molecular Function: ribonucleoprotein binding; protein binding; single-stranded RNA binding; DNA binding; zinc ion binding; double-stranded RNA binding; helicase activity; ATP binding. Biological Process: regulation of apoptosis; protein sumoylation; viral reproduction; detection of virus; response to virus; positive regulation of interferon-beta production; innate immune response; negative regulation of interferon type I production; positive regulation of interferon-alpha production. Disease: Aicardi-goutieres Syndrome 7; Singleton-merten Syndrome 1

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage