Anti-c-SRC (pY529) Antibody | MyBioSource MBS821259 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-c-SRC (pY529) Antibody

catalog :

MBS821259

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, zebrafish

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of c-SRC (pY529) expression in K562 (A), HuvEc (B), Jurkat (C), COLO205 (D), HEK293T EGF-treated (E), A431 pervanadate-treated (F) whole cell lysates.

image 2 :

Immunohistochemical analysis of c-SRC (pY529) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

product information

catalog number :

MBS821259

products type :

Antibody

products full name :

Anti-c-SRC (pY529) Antibody

products short name :

[c-SRC (pY529)]

products name syn :

[SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src]

other names :

[proto-oncogene tyrosine-protein kinase Src; Proto-oncogene tyrosine-protein kinase Src; proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; SRC proto-oncogene, non-receptor tyrosine kinase; Proto-oncogene c-Src; pp60c-src; p60-Src]

products gene name :

[SRC]

other gene names :

[SRC; SRC; ASV; SRC1; c-SRC; p60-Src; SRC1; p60-Src]

uniprot entry name :

SRC_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Chicken, Zebrafish

sequence length :

536

specificity :

Recognizes endogenous levels of c-SRC (pY529) protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human c-SRC. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to c-SRC (pY529)

ncbi gi num :

4885609

ncbi acc num :

NP_005408.1

ncbi gb acc num :

NM_005417.4

uniprot acc num :

P12931

ncbi mol weight :

60,589 Da

ncbi pathways :

ADP Signalling Through P2Y Purinoceptor 1 Pathway (161061); AGE/RAGE Pathway (698754); Adaptive Immune System Pathway (366160); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); AhR Pathway (755436); Alpha-synuclein Signaling Pathway (137913); Alpha6-Beta4 Integrin Signaling Pathway (198807); Androgen Receptor Signaling Pathway (198806); Angiogenesis Pathway (198772)

ncbi summary :

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described. Protein type: EC 2.7.10.2; Protein kinase, TK; Oncoprotein; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Src family. Chromosomal Location of Human Ortholog: 20q12-q13. Cellular Component: neuron projection; mitochondrion; lysosome; postsynaptic density; actin filament; caveola; cytosol; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; late endosome; mitochondrial inner membrane; plasma membrane; nucleus. Molecular Function: protein C-terminus binding; ephrin receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; insulin receptor binding; protein kinase activity; integrin binding; protein binding; enzyme binding; SH3/SH2 adaptor activity; protein kinase C binding; protein-tyrosine kinase activity; heme binding; estrogen receptor binding; kinase activity; SH2 domain binding; ATP binding; receptor binding; hormone receptor binding. Biological Process: oogenesis; regulation of estrogen receptor signaling pathway; central nervous system development; positive regulation of cyclin-dependent protein kinase activity; estrogen receptor signaling pathway; progesterone receptor signaling pathway; nerve growth factor receptor signaling pathway; viral reproduction; regulation of cell cycle; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; uterus development; negative regulation of mitochondrial depolarization; negative regulation of protein homooligomerization; positive regulation of MAP kinase activity; cell-cell adhesion; transforming growth factor beta receptor signaling pathway; cell adhesion; response to electrical stimulus; bone resorption; response to drug; platelet activation; fibroblast growth factor receptor signaling pathway; response to virus; activation of protein kinase B; transcytosis; positive regulation of integrin activation; positive regulation of protein amino acid autophosphorylation; cellular response to insulin stimulus; response to mechanical stimulus; T cell costimulation; regulation of vascular permeability; negative regulation of transcription, DNA-dependent; leukocyte migration; negative regulation of apoptosis; axon guidance; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; negative regulation of caspase activity; positive regulation of smooth muscle cell migration; signal transduction; regulation of cell-cell adhesion; forebrain development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; response to nutrient levels; regulation of bone resorption; negative regulation of focal adhesion formation; positive regulation of phosphoinositide 3-kinase activity; signal complex assembly; response to mineralocorticoid stimulus; cell cycle; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; cell proliferation; peptidyl-serine phosphorylation; response to hydrogen peroxide; regulation of protein binding; Ras protein signal transduction; stress fiber formation; innate immune response; positive regulation of insulin receptor signaling pathway; response to acidity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of cytokine secretion

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage