Anti-Claudin 5 Antibody | MyBioSource MBS821163 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Claudin 5 Antibody

catalog :

MBS821163

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Claudin 5 expression in HeLa (A), mouse liver (B), rat lung (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of Claudin 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Claudin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS821163

products type :

Antibody

products full name :

Anti-Claudin 5 Antibody

products short name :

[Claudin 5]

products name syn :

[AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF]

other names :

[claudin-5; Claudin-5; claudin-5; TMDVCF; transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome; claudin 5; Transmembrane protein deleted in VCFS; TMDVCF]

products gene name :

[CLDN5]

other gene names :

[CLDN5; CLDN5; AWAL; BEC1; TMVCF; CPETRL1; AWAL; TMVCF; TMDVCF]

uniprot entry name :

CLD5_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat, Bovine, Pig, Rabbit

sequence length :

303

specificity :

Recognizes endogenous levels of Claudin 5 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Ships on blue ice. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000); IH (1/100 - 1/200); IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 5. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Claudin 5

ncbi gi num :

195947374

ncbi acc num :

NP_001124333.1

ncbi gb acc num :

NM_001130861.1

uniprot acc num :

O00501

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494)

ncbi summary :

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family. Protein type: Membrane protein, integral; Cytoskeletal; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 22q11.21. Cellular Component: tight junction; plasma membrane; integral to membrane; intercellular junction. Molecular Function: identical protein binding; structural molecule activity. Biological Process: calcium-independent cell-cell adhesion

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage