Anti-FLI1 Antibody | MyBioSource MBS821162 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-FLI1 Antibody

catalog :

MBS821162

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, cow

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of FLI1 expression in HeLa (A), Jurkat (B), mouse liver (C), rat liver (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of FLI1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

product information

catalog number :

MBS821162

products type :

Antibody

products full name :

Anti-FLI1 Antibody

products short name :

[FLI1]

products name syn :

[Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB]

other names :

[Friend leukemia integration 1 transcription factor isoform 2; Friend leukemia integration 1 transcription factor; Friend leukemia integration 1 transcription factor; transcription factor ERGB; Ewing sarcoma breakpoint region 2; Friend leukemia virus integration 1; Fli-1 proto-oncogene, ETS transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB]

products gene name :

[FLI1]

other gene names :

[FLI1; FLI1; EWSR2; SIC-1]

uniprot entry name :

FLI1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Chicken

sequence length :

419

specificity :

Recognizes endogenous levels of FLI1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipping: Blue ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB (1/500 - 1/1000). IH (1/100 - 1/200)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to FLI1

ncbi gi num :

264681554

ncbi acc num :

NP_001161153.1

ncbi gb acc num :

NM_001167681.2

uniprot acc num :

Q01543

ncbi pathways :

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

uniprot summary :

FLI1: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5 -C[CA]GGAAGT-3 . Defects in FLI1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 11q24.1-q24.3. Cellular Component: nucleus. Molecular Function: DNA binding; chromatin binding; transcription factor activity. Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; organ morphogenesis; hemostasis; blood circulation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage