product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-HuB Antibody
catalog :
MBS8211333
quantity :
0.03 mL
price :
155 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, chicken, cow
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of HuB expression in K562 (A), HeLa (B) whole cell lysates.
image 2 :
Immunohistochemical analysis of HuB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
image 3 :
Immunofluorescent analysis of HuB staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
product information
catalog number :
MBS8211333
products type :
Antibody
products full name :
Anti-HuB Antibody
products short name :
[HuB]
products name syn :
[ELAVL2; HUB; ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL4; HUD; PNEM; ELAV-like protein 4; Hu-antigen D; HuD; Paraneoplastic encephalomyelitis antigen HuD]
other names :
[ELAV-like protein 2 isoform b; ELAV-like protein 2; ELAV-like protein 2; ELAV like neuron-specific RNA binding protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1]
products gene name :
[ELAVL2]
products gene name syn :
[ELAVL4]
other gene names :
[ELAVL2; ELAVL2; HUB; HELN1; HEL-N1; HUB; HuB]
uniprot entry name :
ELAV2_HUMAN
clonality :
Polyclonal
host :
Rabbit
reactivity :
Human, Mouse, Bovine, Chicken
sequence length :
346
specificity :
Recognizes endogenous levels of HuB protein.
purity :
The antibody was purified by immunogen affinity chromatography.
form :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
storage stability :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
tested application :
Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
app notes :
WB (1/500 - 1/1000). IHC (1/100 - 1/200). IF (1/100 - 1/500. ICC (1/100 - 1/500)
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Immunofluorescence (IF)
other info1 :
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HuB. The exact sequence is proprietary.
products description :
Rabbit polyclonal antibody to HuB
ncbi gi num :
283945529
ncbi acc num :
NP_001164666.1
ncbi gb acc num :
NM_001171195.1
uniprot acc num :
Q12926
ncbi summary :
The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
uniprot summary :
ELAVL2: Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3 -UTR, the FOS 3 -UTR and the ID 3 -UTR. Belongs to the RRM elav family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding. Chromosomal Location of Human Ortholog: 9p21. Molecular Function: protein binding; mRNA 3 -UTR binding; nucleotide binding. Biological Process: regulation of transcription, DNA-dependent
size1 :
0.03 mL
price1 :
155 USD
size2 :
0.1 mL
price2 :
220
size3 :
0.2 mL
price3 :
295
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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