Anti-ALK1 Antibody | MyBioSource MBS821129 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-ALK1 Antibody

catalog :

MBS821129

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS821129

products type :

Antibody

products full name :

Anti-ALK1 Antibody

products short name :

ALK1

products name syn :

ACVRLK1; Serine/threonine-protein kinase receptor R3; SKR3; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I

other names :

serine/threonine-protein kinase receptor R3; Serine/threonine-protein kinase receptor R3; serine/threonine-protein kinase receptor R3; TGF-B superfamily receptor type I; activin A receptor, type II-like kinase 1; activin A receptor type II-like 1; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I

products gene name :

ACVRL1

other gene names :

ACVRL1; ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1; ACVRLK1; ALK1; SKR3; ALK-1; TSR-I

uniprot entry name :

ACVL1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Dog, Pig

sequence length :

503

specificity :

Recognizes endogenous levels of ALK1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500). Western blot analysis of ALK1 expression in MCF7 (A), mouse liver (B), rat liver (C) whole cell lysates. Immunohistochemical analysis of ALK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of ALK1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

other info1 :

Entrez Gene: 94 (Human); 11482 (Mouse); 25237 (Rat). Swiss Prot: P37023 (Human); Q61288 (Mouse); P80203 (Rat)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ALK1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to ALK1

ncbi gi num :

116734712

ncbi acc num :

NP_000011.2

ncbi gb acc num :

NM_000020.2

uniprot acc num :

P37023

ncbi mol weight :

56,124 Da

ncbi pathways :

ALK1 Pathway (137985); ALK1 Signaling Events Pathway (137968); Id Signaling Pathway (198871); TGF-beta Receptor Signaling Pathway (198774)

ncbi summary :

This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. [provided by RefSeq, Jul 2008]

uniprot summary :

ALK1: a kinase of the STKR family. A type I cell-surface receptor for the TGF-beta superfamily of ligands including Activin A. Highly expressed in human placenta and lung. Deficiency causes hemorrhagic telangiectasia type 2 also known as Rendu-Osler-Weber syndrome 2. Protein type: Protein kinase, TKL; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; Kinase, protein; TKL group; STKR family; Type1 subfamily. Chromosomal Location of Human Ortholog: 12q13.13. Cellular Component: cell surface; cell soma; integral to plasma membrane; dendrite. Molecular Function: metal ion binding; protein kinase binding; transmembrane receptor protein serine/threonine kinase activity; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta binding; transforming growth factor beta receptor activity, type I; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity. Biological Process: protein heterooligomerization; blood circulation; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; negative regulation of cell adhesion; signal transduction; regulation of blood vessel endothelial cell migration; protein amino acid phosphorylation; BMP signaling pathway; negative regulation of cell proliferation; positive regulation of endothelial cell differentiation; negative regulation of endothelial cell differentiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; regulation of blood pressure; lymphangiogenesis; angiogenesis; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of DNA replication; regulation of endothelial cell proliferation; in utero embryonic development; negative regulation of focal adhesion formation; negative regulation of blood vessel endothelial cell migration; blood vessel maturation; blood vessel endothelial cell proliferation during sprouting angiogenesis; positive regulation of angiogenesis; positive regulation of chondrocyte differentiation; negative regulation of endothelial cell proliferation; blood vessel remodeling; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; wound healing, spreading of epidermal cells. Disease: Telangiectasia, Hereditary Hemorrhagic, Type 2

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage