Anti-Collagen 2 alpha 1 Antibody | MyBioSource MBS8210940 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Collagen 2 alpha 1 Antibody

catalog :

MBS8210940

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Collagen 2 alpha 1 expression in MCF7 (A) whole cell lysates.

image 2 :

Immunohistochemical analysis of Collagen 2 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

product information

catalog number :

MBS8210940

products type :

Antibody

products full name :

Anti-Collagen 2 alpha 1 Antibody

products short name :

[Collagen 2 alpha 1]

products name syn :

[Collagen alpha-1(II) chain; Alpha-1 type II collagen]

other names :

[collagen alpha-1(II) chain isoform 1; Collagen alpha-1(II) chain; collagen alpha-1(II) chain; collagen, type II, alpha 1; Alpha-1 type II collagen]

products gene name :

[COL2A1]

other gene names :

[COL2A1; COL2A1; AOM; ANFH; SEDC; STL1; COL11A3]

uniprot entry name :

CO2A1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Dog, Porcine, Rabbit

sequence length :

268

specificity :

Recognizes endogenous levels of Collagen 2 alpha 1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Ships on blue ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB (1/500 - 1/1000). IHC (1/100 - 1/200)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 2 alpha 1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Collagen 2 alpha 1

ncbi gi num :

111118976

ncbi acc num :

NP_001835.3

ncbi gb acc num :

NM_001844.4

uniprot acc num :

P02458

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1270247); Collagen Biosynthesis And Modifying Enzymes Pathway (1270246); Collagen Formation Pathway (1270245); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (1270244); Focal Adhesion Pathway (198795)

ncbi summary :

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 12q13.11. Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type II; extracellular region; basement membrane. Molecular Function: identical protein binding; metal ion binding; platelet-derived growth factor binding; extracellular matrix structural constituent conferring tensile strength. Biological Process: heart morphogenesis; proteoglycan metabolic process; axon guidance; extracellular matrix organization and biogenesis; inner ear morphogenesis; central nervous system development; collagen fibril organization; palate development; notochord development; collagen catabolic process; extracellular matrix disassembly; limb bud formation; sensory perception of sound; visual perception; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; skeletal development; endochondral ossification; cartilage condensation. Disease: Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Achondrogenesis, Type Ii; Kniest Dysplasia; Otospondylomegaepiphyseal Dysplasia; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Spondyloepiphyseal Dysplasia Congenita; Avascular Necrosis Of Femoral Head, Primary; Legg-calve-perthes Disease; Spondyloperipheral Dysplasia; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Osteoarthritis With Mild Chondrodysplasia; Stickler Syndrome, Type I; Czech Dysplasia; Stickler Syndrome, Type I, Nonsyndromic Ocular

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage