Anti-p15 INK4b Antibody | MyBioSource MBS821044 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-p15 INK4b Antibody

catalog :

MBS821044

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of p15 INK4b expression in HepG2 (A), A549 (B), HeLa (C), mouse brain (D), rat kidney (E) whole cell lysates.

image 2 :

Immunohistochemical analysis of p15 INK4b staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of p15 INK4b staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS821044

products type :

Antibody

products full name :

Anti-p15 INK4b Antibody

products short name :

[p15 INK4b]

products name syn :

[MTS2; Cyclin-dependent kinase 4 inhibitor B; Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b; p15INK4B]

other names :

[cyclin-dependent kinase 4 inhibitor B isoform 1; Cyclin-dependent kinase 4 inhibitor B; cyclin-dependent kinase 4 inhibitor B; MTS-2; p14-INK4b; p14_INK4B; p15-INK4b; p15_INK4B; CDK4B inhibitor; p14_CDK inhibitor; p15 CDK inhibitor; CDK inhibitory protein; multiple tumor suppressor 2; cyclin-dependent kinases 4 and 6 binding protein; cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4); Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b; p15INK4B]

products gene name :

[CDKN2B]

other gene names :

[CDKN2B; CDKN2B; P15; MTS2; TP15; CDK4I; INK4B; p15INK4b; MTS2; MTS-2; p15INK4B]

uniprot entry name :

CDN2B_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Monkey, Pig

sequence length :

138

specificity :

Recognizes endogenous levels of p15 INK4b protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipping: Blue ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000). IH (1/100 - 1/200). IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p15 INK4b. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to p15 INK4b

ncbi gi num :

17981694

ncbi acc num :

NP_004927.2

ncbi gb acc num :

NM_004936.3

uniprot acc num :

P42772

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Cyclin D Associated Events In G1 Pathway (105767); Disease Pathway (530764); FoxO Signaling Pathway (921162); G1 Phase Pathway (105766)

ncbi summary :

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

uniprot summary :

p15-INK4B: a potent inhibitor that nteracts strongly with CDK4 and CDK6. Potential effector of TGF-beta induced cell cycle arrest. Protein type: Inhibitor; Cell cycle regulation; Protein kinase, regulatory subunit; Tumor suppressor. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus. Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding. Biological Process: spleen development; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; megakaryocyte differentiation; mitotic cell cycle checkpoint; positive regulation of transforming growth factor beta receptor signaling pathway; cellular response to nutrient; liver development; response to organic cyclic substance; negative regulation of cell proliferation; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; cellular response to extracellular stimulus; negative regulation of phosphorylation; positive regulation of epithelial cell differentiation; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; cell cycle arrest; aging; negative regulation of epithelial cell proliferation

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage