Anti-CD35 Antibody-FITC labled | MyBioSource MBS8209536 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CD35 Antibody-FITC labled

catalog :

MBS8209536

quantity :

50 Assays

price :

215 USD

clonality :

monoclonal

host :

mouse

conjugate :

FITC

reactivity :

human

application :

immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Flow cytometric analysis of human peripheral blood granulocytes using Anti-CD35 Antibody-FITC labled.

product information

catalog number :

MBS8209536

products type :

Antibody

products full name :

Anti-CD35 Antibody-FITC labled

products short name :

[CD35]

products name syn :

[C3BR; Complement receptor type 1; C3b/C4b receptor; CD antigen CD35]

other names :

[complement receptor type 1 isoform F; Complement receptor type 1; complement receptor type 1; complement component (3b/4b) receptor 1 (Knops blood group); C3b/C4b receptor; CD_antigen: CD35]

products gene name :

[CR1]

other gene names :

[CR1; CR1; KN; C3BR; C4BR; CD35; C3BR]

uniprot entry name :

CR1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

host :

Mouse

reactivity :

Human

sequence length :

2039

specificity :

Recognises human CD35.

purity :

The antibody was purified by affinity chromatography.

form :

Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.

storage stability :

Shipped and store at 4 degree C for one year. Do not freeze.

tested application :

Immunofluoresence (IF), Flow Cytometry (FC/FACS)

image1 heading :

Flow Cytometry (FC/FACS)

other info1 :

Conjugation: FITC

other info2 :

Directions for Use: 1. Take 100 ul peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 ul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant.

products description :

Mouse monoclonal antibody to CD35 (FITC)

ncbi gi num :

86793036

ncbi acc num :

NP_000564.2

ncbi gb acc num :

NM_000573.3

uniprot acc num :

P17927

ncbi mol weight :

223,663 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1269241); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Legionellosis Pathway (469200); Legionellosis Pathway (469186)

ncbi summary :

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

CR1: Mediates cellular binding of particles and immune complexes that have activated complement. Belongs to the receptors of complement activation (RCA) family. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: cell surface; integral to plasma membrane; plasma membrane. Molecular Function: complement component C3b receptor activity; complement component C4b binding; complement component C4b receptor activity; complement component C3b binding. Biological Process: negative regulation of complement activation, alternative pathway; complement receptor mediated signaling pathway; negative regulation of complement activation, classical pathway; regulation of complement activation; innate immune response; complement activation, classical pathway. Disease: Knops Blood Group System; Malaria, Susceptibility To

size1 :

50 Assays

price1 :

215 USD

size2 :

100 Assays

price2 :

280

more info or order :

MyBioSource product webpage