Anti-PAR1 Antibody | MyBioSource MBS820869 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-PAR1 Antibody

catalog :

MBS820869

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of PAR1 expression in PaCa2 (A), HeLa (B), TF1 (C), ECV304 (D) whole cell lysates.

image 2 :

Immunofluorescent analysis of PAR1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS820869

products type :

Antibody

products full name :

Anti-PAR1 Antibody

products short name :

[PAR1]

products name syn :

[CF2R; PAR1; TR; Proteinase-activated receptor 1; PAR-1; Coagulation factor II receptor; Thrombin receptor]

other names :

[proteinase-activated receptor 1; Proteinase-activated receptor 1; proteinase-activated receptor 1; protease-activated receptor 1; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; Thrombin receptor]

products gene name :

[F2R]

other gene names :

[F2R; F2R; TR; HTR; CF2R; PAR1; PAR-1; CF2R; PAR1; TR; PAR-1]

uniprot entry name :

PAR1_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human

sequence length :

425

specificity :

Recognizes endogenous levels of PAR1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

WB, IF/ IC

app notes :

WB (1/500-1/1000). IF/IC (1/100-1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info2 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PAR1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to PAR1

ncbi gi num :

166362740

ncbi acc num :

NP_001983.2

ncbi gb acc num :

NM_001992.3

uniprot acc num :

P25116

ncbi pathways :

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Endocytosis Pathway (102279); Endocytosis Pathway (102181); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548)

ncbi summary :

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008]

uniprot summary :

PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development. Protein type: Receptor, GPCR; GPCR, family 1; Cell development/differentiation; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 5q13. Cellular Component: Golgi apparatus; postsynaptic membrane; integral to plasma membrane; early endosome; extracellular region; plasma membrane; caveola; neuromuscular junction. Molecular Function: G-protein coupled receptor activity; protein binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; thrombin receptor activity; receptor binding. Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of blood coagulation; negative regulation of glomerular filtration; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; positive regulation of JAK-STAT cascade; positive regulation of caspase activity; response to lipopolysaccharide; negative regulation of cell proliferation; connective tissue replacement during inflammatory response; elevation of cytosolic calcium ion concentration; platelet dense granule organization and biogenesis; positive regulation of MAPKKK cascade; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of cell proliferation; response to wounding; positive regulation of smooth muscle contraction; negative regulation of neuron apoptosis; inflammatory response; establishment of synaptic specificity at neuromuscular junction; STAT protein nuclear translocation; caspase activation; platelet activation; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of Rho protein signal transduction; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; regulation of blood coagulation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of release of sequestered calcium ion into cytosol; regulation of sensory perception of pain; blood coagulation; positive regulation of calcium ion transport; positive regulation of cell migration

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage