Anti-SMG7 Antibody | MyBioSource MBS820862 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-SMG7 Antibody

catalog :

MBS820862

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of SMG7 expression in Jurkat (A), A431 (B), HeLa (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of SMG7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of SMG7 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS820862

products type :

Antibody

products full name :

Anti-SMG7 Antibody

products short name :

[SMG7]

products name syn :

[C1orf16; EST1C; KIAA0250; Protein SMG7; EST1-like protein C; SMG-7 homolog; hSMG-7]

other names :

[protein SMG7 isoform 5; Protein SMG7; protein SMG7; EST1-like protein C; ever shorter telomeres 1C; EST1 telomerase component homolog C; breast cancer-associated antigen SGA-56M; smg-7 homolog, nonsense mediated mRNA decay factor; SMG7 nonsense mediated mRNA decay factor; EST1-like protein C; SMG-7 homolog; hSMG-7]

products gene name :

[SMG7]

other gene names :

[SMG7; SMG7; EST1C; SGA56M; C1orf16; C1orf16; EST1C; KIAA0250; hSMG-7]

uniprot entry name :

SMG7_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

1145

specificity :

Recognizes endogenous levels of SMG7 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipping: Blue Ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IH), Immunofluorescence/Immunocytochemistry (IF/IC), Immunoprecipitation (IP)

app notes :

WB (1/500 - 1/1000). IH (1/100 - 1/200). IF/IC (1/100 - 1/500). IP (1/10 - 1/100)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMG7. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to SMG7

ncbi gi num :

291327485

ncbi acc num :

NP_001167532.1

ncbi gb acc num :

NM_001174061.1

uniprot acc num :

Q92540

ncbi pathways :

Gene Expression Pathway (105937); Nonsense Mediated Decay (NMD) Enhanced By The Exon Junction Complex (EJC) Pathway (366241); Nonsense-Mediated Decay (NMD) Pathway (366240); MRNA Surveillance Pathway (193328); MRNA Surveillance Pathway (193184)

ncbi summary :

This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

uniprot summary :

SMG7: Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 1q25. Cellular Component: intermediate filament cytoskeleton; cytoplasm; nucleus; cytosol. Molecular Function: protein binding; protein phosphatase 2A binding. Biological Process: mRNA export from nucleus; mRNA catabolic process, nonsense-mediated decay; regulation of dephosphorylation; gene expression

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage