NR0B1 siRNA (Human) | MyBioSource MBS8208186 product information

product summary

company name :

MyBioSource

product type :

gene knockdown

product name :

NR0B1 siRNA (Human)

catalog :

MBS8208186

quantity :

15 nmol

price :

365 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS8208186

products type :

siRNA

products full name :

NR0B1 siRNA (Human)

products short name :

[NR0B1]

products name syn :

[AHC; DAX1; Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1]

other names :

[nuclear receptor subfamily 0 group B member 1; Nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0, group B, member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1]

products gene name :

[NR0B1]

other gene names :

[NR0B1; NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2; AHC; DAX1]

uniprot entry name :

NR0B1_HUMAN

reactivity :

Human

sequence length :

470

specificity :

NR0B1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

purity :

> 97%

form :

Lyophilized powder

storage stability :

Shipped at 4 degree C. Store at -20 degree C for one year.

tested application :

RNA Interference (RNAi)

other info2 :

Source: Synthetic. Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NR0B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

products description :

siRNA to inhibit NR0B1 expression using RNA interference

ncbi gi num :

5016090

ncbi acc num :

NP_000466.2

ncbi gb acc num :

NM_000475.4

uniprot acc num :

P51843

ncbi mol weight :

43,590 Da

ncbi pathways :

Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Nuclear Receptor Transcription Pathway (1269652); Nuclear Receptors Pathway (198848); Regulation Of Androgen Receptor Activity Pathway (138027)

ncbi summary :

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, Jul 2008]

uniprot summary :

NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described. Protein type: Nuclear receptor. Chromosomal Location of Human Ortholog: Xp21.3. Cellular Component: nucleoplasm; polysomal ribosome; membrane; cytoplasm; nucleus. Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; RNA binding; transcription factor binding; steroid hormone receptor binding; DNA hairpin binding; protein binding; DNA binding; AF-2 domain binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription corepressor activity. Biological Process: transcription initiation from RNA polymerase II promoter; negative regulation of steroid hormone receptor signaling pathway; hypothalamus development; gonad development; intracellular receptor-mediated signaling pathway; adrenal gland development; negative regulation of transcription factor activity; male gonad development; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell differentiation; protein localization; Leydig cell differentiation; pituitary gland development; male sex determination; steroid hormone mediated signaling; gene expression; spermatogenesis; negative regulation of transcription, DNA-dependent; steroid biosynthetic process. Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital

size1 :

15 nmol

price1 :

365 USD

size2 :

30 nmol

price2 :

515

more info or order :

MyBioSource product webpage