Anti-Histamine H2 Receptor Antibody | MyBioSource MBS820672 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Histamine H2 Receptor Antibody

catalog :

MBS820672

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS820672

products type :

Antibody

products full name :

Anti-Histamine H2 Receptor Antibody

products short name :

Histamine H2 Receptor

products name syn :

Histamine H2 receptor; H2R; HH2R; Gastric receptor I

other names :

histamine H2 receptor isoform 1; Histamine H2 receptor; histamine H2 receptor; HH2R; gastric receptor 1; gastric receptor I; histamine receptor H2; Gastric receptor I

products gene name :

HRH2

other gene names :

HRH2; Hrh2; H2R; H2R; HH2R

uniprot entry name :

HRH2_MOUSE

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

397

specificity :

Recognizes endogenous levels of Histamine H2 Receptor protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)

app notes :

WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500). Western blot analysis of Histamine H2 Receptor expression in HEK293T (A), COLO205 (B), NIH3T3 (C), H9C2 (D) whole cell lysates. Immunofluorescent analysis of Histamine H2 Receptor staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H2 Receptor. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Histamine H2 Receptor

ncbi gi num :

197085610

ncbi acc num :

NP_001124527.1

ncbi gb acc num :

NM_001131055.1

uniprot acc num :

P25021

ncbi mol weight :

44,794 Da

ncbi pathways :

Amine Ligand-binding Receptors Pathway (106363); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383)

ncbi summary :

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 5q35.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: histamine receptor activity. Biological Process: gland development; G-protein signaling, coupled to cyclic nucleotide second messenger; gastrin-induced gastric acid secretion; gut development; histamine-induced gastric acid secretion; positive regulation of vasoconstriction; visual learning; regulation of synaptic plasticity; immune response; memory

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage