Anti-HOXA11/D11 Antibody | MyBioSource MBS820585 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-HOXA11/D11 Antibody

catalog :

MBS820585

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, chicken

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of HOXA11/D11 expression in HEK293T (A), HeLa (B), K562 (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of HOXA11/D11 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of HOXA11/D11 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS820585

products type :

Antibody

products full name :

Anti-HOXA11/D11 Antibody

products short name :

[HOXA11/D11]

products name syn :

[HOXA11; HOX1I; Homeobox protein Hox-A11; Homeobox protein Hox-1I; HOXD11; HOX4F; Homeobox protein Hox-D11; Homeobox protein Hox-4F]

other names :

[homeobox protein Hox-A11; Homeobox protein Hox-A11; homeobox protein Hox-A11; homeo box 1I; homeobox protein HOXA11; homeobox protein Hox-1I; homeobox A11; Homeobox protein Hox-1I]

products gene name :

[HOXA11]

products gene name syn :

[HOXD11]

other gene names :

[HOXA11; HOXA11; HOX1; HOX1I; HOX1I]

uniprot entry name :

HXA11_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Chicken

sequence length :

313

specificity :

Recognizes endogenous levels of HOXA11/D11 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HOXA11/D11. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to HOXA11/D11

ncbi gi num :

5031759

ncbi acc num :

NP_005514.1

ncbi gb acc num :

NM_005523.5

uniprot acc num :

P31270

ncbi mol weight :

34,486 Da

ncbi pathways :

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is involved in the regulation of uterine development and is required for female fertility. Mutations in this gene can cause radio-ulnar synostosis with amegakaryocytic thrombocytopenia. [provided by RefSeq, Jul 2008]

uniprot summary :

HOXA11: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Defects in HOXA11 are the cause of radioulnar synostosis with amegakaryocytic thrombocytopenia (RSAT). The syndrome consists of an unusual association of bone marrow failure and skeletal defects. Patients have the same skeletal defects, the proximal fusion of the radius and ulna, resulting in extremely limited pronation and supination of the forearm. Some patients have also symptomatic thrombocytopenia, with bruising and bleeding problems since birth, necessitating correction by bone marrow or umbilical-cord stem-cell transplantation. Belongs to the Abd-B homeobox family. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 7p15.2. Cellular Component: transcription factor complex; protein complex; nucleus. Molecular Function: sequence-specific DNA binding. Biological Process: embryonic forelimb morphogenesis; developmental growth; anatomical structure morphogenesis; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription, DNA-dependent; male gonad development; uterus development; anterior/posterior pattern formation; positive regulation of chondrocyte differentiation; dorsal/ventral pattern formation; ureteric bud branching; single fertilization; induction of an organ; mesodermal cell fate specification; spermatogenesis; embryonic digit morphogenesis; skeletal development; metanephros development; proximal/distal pattern formation; embryonic limb morphogenesis. Disease: Radioulnar Synostosis With Amegakaryocytic Thrombocytopenia

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage