antibody

Anti-JIP3 Antibody

MBS820556

0.03 mL

155 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, cow

western blot, immunohistochemistry, immunocytochemistry

Antibody

Anti-JIP3 Antibody

JIP3

JIP3; KIAA1066; C-Jun-amino-terminal kinase-interacting protein 3; JIP-3; JNK-interacting protein 3; JNK MAP kinase scaffold protein 3; Mitogen-activated protein kinase 8-interacting protein 3

C-Jun-amino-terminal kinase-interacting protein 3 isoform 2; C-Jun-amino-terminal kinase-interacting protein 3; C-Jun-amino-terminal kinase-interacting protein 3; JIP-3; JNK-interacting protein 3; JNK/SAPK-associated protein-1; JNK MAP kinase scaffold protein 3; homolog of Drosophila Sunday driver 2; C-jun-amino-terminal kinase interacting protein 3; JNK/stress-activated protein kinase-associated protein 1; mitogen-activated protein kinase 8-interacting protein 3; mitogen-activated protein kinase 8 interacting protein 3; JNK MAP kinase scaffold protein 3; Mitogen-activated protein kinase 8-interacting protein 3

MAPK8IP3

MAPK8IP3; MAPK8IP3; syd; JIP3; SYD2; JSAP1; JIP3; KIAA1066; JIP-3; JNK-interacting protein 3

JIP3_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat, Bovine

1330

Recognizes endogenous levels of JIP3 protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500). Western blot analysis of JIP3 expression in IMR32 (A), mouse brain.rat brain (B), PC12 (C) whole cell lysates. Immunohistochemical analysis of JIP3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of JIP3 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Entrez Gene: 23162 (Human); 30954 (Mouse). SwissProt: Q9UPT6 (Human); Q9ESN9 (Mouse)

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human JIP3. The exact sequence is proprietary.

Rabbit polyclonal antibody to JIP3

94557295

NP_001035529.1

NM_001040439.1

Q9UPT6

29,052 Da

Arf6 Trafficking Events Pathway (137954); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Signaling Events Mediated By Focal Adhesion Kinase Pathway (169348)

The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

JIP3: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies in the mouse suggested that this protein may interact with and regulate the activity of numerous protein kinases of the JNK signaling pathway. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: Golgi membrane; smooth endoplasmic reticulum; dendrite; cytoplasm; axolemma. Molecular Function: JUN kinase binding; MAP-kinase scaffold activity; receptor signaling complex scaffold activity; mitogen-activated protein kinase kinase binding; kinesin binding; mitogen-activated protein kinase kinase kinase binding. Biological Process: vesicle-mediated transport; axon guidance; protein localization; regulation of gene expression; in utero embryonic development; forebrain development; respiratory gaseous exchange; positive regulation of neuron differentiation; alveolus development; regulation of JNK cascade; activation of JNK activity; post-embryonic development

0.03 mL

155 USD

0.1 mL

220

0.2 mL

295