Anti-A20 Antibody | MyBioSource MBS820465 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-A20 Antibody

catalog :

MBS820465

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of A20 expression in HeLa (A), Daudi (B), RAW264.7 (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of A20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of A20 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS820465

products type :

Antibody

products full name :

Anti-A20 Antibody

products short name :

[A20]

products name syn :

[OTUD7C; Tumor necrosis factor alpha-induced protein 3; TNF alpha-induced protein 3; OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20]

other names :

[tumor necrosis factor alpha-induced protein 3; Tumor necrosis factor alpha-induced protein 3; tumor necrosis factor alpha-induced protein 3; zinc finger protein A20; TNF alpha-induced protein 3; OTU domain-containing protein 7C; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha-induced protein 3; OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20Cleaved into the following 2 chains:A20p50; A20p37]

products gene name :

[TNFAIP3]

other gene names :

[TNFAIP3; TNFAIP3; A20; OTUD7C; TNFA1P2; OTUD7C; TNF alpha-induced protein 3]

uniprot entry name :

TNAP3_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

790

specificity :

Recognizes endogenous levels of A20 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipping:Blue Ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000). IH (1/100 - 1/200). IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human A20. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to A20

ncbi gi num :

395393995

ncbi acc num :

NP_001257436.1

ncbi gb acc num :

NM_001270507.1

uniprot acc num :

P21580

ncbi pathways :

CD40/CD40L Signaling Pathway (138061); Canonical NF-kappaB Pathway (138030); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Immune System Pathway (106386); Innate Immune System Pathway (106387); Measles Pathway (213306); Measles Pathway (213277); NF-kappa B Signaling Pathway (634527); NOD-like Receptor Signaling Pathway (122191)

ncbi summary :

This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]

uniprot summary :

TNFAIP3: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates Lys-63 -polyubiquitin chains on RIPK1 and catalyzes the formation of Lys-48 -polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on Lys-63 -linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates Lys-63 -polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both Lys-48 - and Lys-63 polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. By TNF. Belongs to the peptidase C64 family. Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Apoptosis; Protease. Chromosomal Location of Human Ortholog: 6q23. Cellular Component: centrosome; lysosome; cytoplasm; nucleus; cytosol. Molecular Function: ubiquitin binding; protein binding; protease binding; DNA binding; protein self-association; zinc ion binding; ubiquitin-specific protease activity; ubiquitin-protein ligase activity; kinase binding; ligase activity. Biological Process: negative regulation of toll-like receptor 5 signaling pathway; negative regulation of smooth muscle cell proliferation; apoptosis; proteolysis; negative regulation of toll-like receptor 3 signaling pathway; negative regulation of interleukin-2 production; negative regulation of innate immune response; response to molecule of bacterial origin; negative regulation of interleukin-6 production; inflammatory response; negative regulation of bone resorption; negative regulation of chronic inflammatory response; protein deubiquitination; negative regulation of toll-like receptor 2 signaling pathway; negative regulation of cyclin-dependent protein kinase activity; B-1 B cell homeostasis; negative regulation of B cell activation; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta production; regulation of germinal center formation; protein oligomerization; inhibition of NF-kappaB transcription factor; response to muramyl dipeptide; negative regulation of inflammatory response; negative regulation of toll-like receptor 4 signaling pathway; positive regulation of protein catabolic process; innate immune response; negative regulation of protein ubiquitination; regulation of defense response to virus by host; negative regulation of interferon type I production

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage