Anti-CD326 Antibody | MyBioSource MBS8204495 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CD326 Antibody

catalog :

MBS8204495

quantity :

0.05 mL

price :

175 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Flow cytometric analysis of TF1 cells using Anti-CD326 Antibody, followed by anti-mouse IgG PE.

product information

catalog number :

MBS8204495

products type :

Antibody

products full name :

Anti-CD326 Antibody

products short name :

[CD326]

products name syn :

[GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD antigen CD326]

other names :

[epithelial cell adhesion molecule; Epithelial cell adhesion molecule; epithelial cell adhesion molecule; epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD_antigen: CD326]

products gene name :

[EPCAM]

other gene names :

[EPCAM; EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314]

uniprot entry name :

EPCAM_HUMAN

clonality :

Monoclonal

isotype :

IgG1

host :

Mouse

reactivity :

Human

sequence length :

314

specificity :

Recognises human CD326.

purity :

The antibody was purified by affinity chromatography.

form :

Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.

storage stability :

Shipping: Blue ice . Store at 4°C for one year. Do not freeze.

tested application :

Immunofluoresence (IF), Flow Cytometry (FC/FACS)

image1 heading :

Flow Cytometry (FC/FACS)

other info2 :

Directions for use: 1.- Take 100 ul peripheral blood anticoagulated by EDTA and add to the bottom of 5mL tube. 2.- Add aproppiate amount of antibody to the bottom of flow tube mixing with the blood; incubate for 30 minutes at room temperature. 3.- Add 2mL RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4.- Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5.- Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6.- Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7.- Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4°C then measured).

products description :

Mouse monoclonal antibody to CD326

ncbi gi num :

218505670

ncbi acc num :

NP_002345.2

ncbi gb acc num :

NM_002354.2

uniprot acc num :

P16422

ncbi summary :

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

uniprot summary :

EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2p21. Cellular Component: cell surface; tight junction; basolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; lateral plasma membrane. Molecular Function: protein binding; protein complex binding. Biological Process: ureteric bud development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation. Disease: Diarrhea 5, With Tufting Enteropathy, Congenital; Colorectal Cancer, Hereditary Nonpolyposis, Type 8

size1 :

0.05 mL

price1 :

175 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage