Anti-Beta-glucosidase 2 Antibody | MyBioSource MBS820370 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Beta-glucosidase 2 Antibody

catalog :

MBS820370

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Beta-glucosidase 2 expression in A431 (A), HeLa (B), A549 (C) whole cell lysates.

image 2 :

Immunofluorescent analysis of Beta-glucosidase 2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS820370

products type :

Antibody

products full name :

Anti-Beta-glucosidase 2 Antibody

products short name :

[Beta-glucosidase 2]

products name syn :

[G19P1; Glucosidase 2 subunit beta; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH]

other names :

[glucosidase 2 subunit beta isoform 2; Glucosidase 2 subunit beta; glucosidase 2 subunit beta; hepatocystin; AGE-binding receptor 2; glucosidase II subunit beta; protein kinase C substrate, 80 Kda protein; protein kinase C substrate 60.1 kDa protein heavy chain; protein kinase C substrate 80K-H; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH]

products gene name :

[PRKCSH]

other gene names :

[PRKCSH; PRKCSH; PCLD; PLD1; G19P1; PKCSH; AGE-R2; G19P1; PKCSH]

uniprot entry name :

GLU2B_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human

sequence length :

525

specificity :

Recognizes endogenous levels of Beta-glucosidase 2 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)

app notes :

WB (1/500 - 1/1000); IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info2 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Beta-glucosidase 2. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Beta-glucosidase 2

ncbi gi num :

48255891

ncbi acc num :

NP_001001329.1

ncbi gb acc num :

NM_001001329.2

uniprot acc num :

P14314

ncbi mol weight :

59,178 Da

ncbi pathways :

Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Asparagine N-linked Glycosylation Pathway (161013); Calnexin/calreticulin Cycle Pathway (187218); Immune System Pathway (106386); Innate Immune System Pathway (106387); Metabolism Of Proteins Pathway (106230); N-glycan Trimming In The ER And Calnexin/Calreticulin Cycle Pathway (187217); Post-translational Protein Modification Pathway (161012); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)

ncbi summary :

This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

uniprot summary :

PRKCSH: Regulatory subunit of glucosidase II. Defects in PRKCSH are a cause of polycystic liver disease (PCLD). PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD), which in a considerable but uncertain proportion of cases is associated with hepatic cysts. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: endoplasmic reticulum lumen; endoplasmic reticulum; intracellular. Molecular Function: protein kinase C binding; phosphoprotein binding; calcium ion binding. Biological Process: cellular protein metabolic process; protein folding; innate immune response; protein amino acid N-linked glycosylation via asparagine; N-glycan processing; post-translational protein modification. Disease: Polycystic Liver Disease

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage