Anti-Claudin 2 Antibody | MyBioSource MBS820337 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Claudin 2 Antibody

catalog :

MBS820337

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Claudin 2 expression in HeLa (A), A431 (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of Claudin 2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Claudin 2 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS820337

products type :

Antibody

products full name :

Anti-Claudin 2 Antibody

products short name :

[Claudin 2]

products name syn :

[Claudin-2; SP82]

other names :

[claudin-2; Claudin-2; claudin-2; SP82; claudin 2; SP82]

products gene name :

[CLDN2]

other gene names :

[CLDN2; CLDN2]

uniprot entry name :

CLD2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Dog, Pig

sequence length :

230

specificity :

Recognizes endogenous levels of Claudin 2 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C -term reg ion of human Claudin 2. The exact sequence is proprietary

products description :

Rabbit polyclonal antibody to Claudin 2

ncbi gi num :

283806628

ncbi acc num :

NP_001164563.1

ncbi gb acc num :

NM_001171092.1

uniprot acc num :

P57739

ncbi mol weight :

24,549 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Tight Junction Pathway (83071)

ncbi summary :

This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]

uniprot summary :

Claudin-2: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family. Protein type: Cytoskeletal; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: Xq22.3-q23. Cellular Component: tight junction; plasma membrane; integral to membrane. Molecular Function: identical protein binding; structural molecule activity. Biological Process: calcium-independent cell-cell adhesion

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage