Anti-Galectin 9 Antibody | MyBioSource MBS820334 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Galectin 9 Antibody

catalog :

MBS820334

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Galectin 9 expression in THP1 (A), mouse spleen (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of Galectin 9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Galectin 9 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS820334

products type :

Antibody

products full name :

Anti-Galectin 9 Antibody

products short name :

[Galectin 9]

products name syn :

[Galectin-9; Gal-9; Ecalectin; Tumor antigen HOM-HD-21]

other names :

[galectin-9 isoform short; Galectin-9; galectin-9; gal-9; ecalectin; galectin 9; tumor antigen HOM-HD-21; urate transporter/channel protein; lectin, galactoside-binding, soluble, 9; Ecalectin; Tumor antigen HOM-HD-21]

products gene name :

[LGALS9]

other gene names :

[LGALS9; LGALS9; HUAT; LGALS9A; Gal-9]

uniprot entry name :

LEG9_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human

sequence length :

323

specificity :

Recognizes endogenous levels of Galectin 9 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Galectin 9. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Galectin 9

ncbi gi num :

102469694

ncbi acc num :

NP_002299.2

ncbi gb acc num :

NM_002308.3

uniprot acc num :

O00182

ncbi mol weight :

35,888 Da

ncbi summary :

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Unknown function. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: cytoplasm. Molecular Function: signal transducer activity; galactose binding; carbohydrate binding. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage