Anti-mGLUR5 Antibody | MyBioSource MBS820275 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-mGLUR5 Antibody

catalog :

MBS820275

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of mGLUR5 expression in EOC20 (A), mouse liver (B), rat liver (C), rat brain (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of mGLUR5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of mGLUR5 staining in EOC20 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS820275

products type :

Antibody

products full name :

Anti-mGLUR5 Antibody

products short name :

[mGLUR5]

products name syn :

[GPRC1E; MGLUR5; Metabotropic glutamate receptor 5; mGluR5]

other names :

[metabotropic glutamate receptor 5 isoform b; Metabotropic glutamate receptor 5; metabotropic glutamate receptor 5; protein phosphatase 1, regulatory subunit 86; glutamate receptor, metabotropic 5]

products gene name :

[GRM5]

other gene names :

[GRM5; GRM5; mGlu5; GPRC1E; MGLUR5; PPP1R86; GPRC1E; MGLUR5; mGluR5]

uniprot entry name :

GRM5_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Dog, Monkey, Pig

sequence length :

1180

specificity :

Recognizes endogenous levels of mGLUR5 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human mGLUR5. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to mGLUR5

ncbi gi num :

4504143

ncbi acc num :

NP_000833.1

ncbi gb acc num :

NM_000842.4

uniprot acc num :

P41594

ncbi pathways :

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway (106381); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway (198839); Gap Junction Pathway (83072); Gap Junction Pathway (483); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295)

ncbi summary :

This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

uniprot summary :

mGluR5: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system and generates a calcium-activated chloride current. Belongs to the G-protein coupled receptor 3 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 3; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 11q14.3. Cellular Component: integral to plasma membrane; cytoplasm; postsynaptic density; plasma membrane; dendritic spine; dendritic shaft. Molecular Function: G-protein coupled receptor activity; glutamate receptor activity; A2A adenosine receptor binding. Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; regulation of synaptic transmission, glutamatergic; locomotory behavior; learning; protein amino acid phosphorylation; positive regulation of long-term neuronal synaptic plasticity; synaptic transmission; negative regulation of locomotion; regulation of transcription, DNA-dependent; metabotropic glutamate receptor, phospholipase C activating pathway; protein kinase C activation; metabotropic glutamate receptor signaling pathway; activation of MAPKKK activity; cognition

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage