Anti-Laminin beta 1 Antibody | MyBioSource MBS8202420 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Laminin beta 1 Antibody

catalog :

MBS8202420

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Laminin beta 1 expression in A431 (A), SW480 (B), PC3 (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of Laminin beta 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

product information

catalog number :

MBS8202420

products type :

Antibody

products full name :

Anti-Laminin beta 1 Antibody

products short name :

[Laminin beta 1]

products name syn :

[Laminin subunit beta-1; Laminin B1 chain; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta]

other names :

[laminin subunit beta-1; Laminin subunit beta-1; laminin subunit beta-1; laminin, beta 1; Laminin B1 chain; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta]

products gene name :

[LAMB1]

other gene names :

[LAMB1; LAMB1; CLM; LIS5]

uniprot entry name :

LAMB1_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human

sequence length :

1786

specificity :

Recognizes endogenous levels of Laminin beta 1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB (1/500 - 1/1000), IHC (1/100 - 1/200)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Laminin beta 1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Laminin beta 1

ncbi gi num :

167614504

ncbi acc num :

NP_002282.2

ncbi gb acc num :

NM_002291.2

uniprot acc num :

P07942

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Axon Guidance Pathway (1270303); Degradation Of The Extracellular Matrix Pathway (1270257); Developmental Biology Pathway (1270302); ECM Proteoglycans Pathway (1270256); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1270244)

ncbi summary :

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq, Aug 2011]

uniprot summary :

LAMB1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: extracellular matrix; extracellular space; laminin-8 complex; laminin-1 complex; laminin-2 complex; perinuclear region of cytoplasm; laminin-10 complex; extracellular region; basement membrane. Molecular Function: integrin binding; enzyme binding; extracellular matrix structural constituent; structural molecule activity; glycosphingolipid binding. Biological Process: odontogenesis; axon guidance; extracellular matrix disassembly; extracellular matrix organization and biogenesis; neuronal-glial interaction involved in cerebral cortex radial glia guided migration; negative regulation of cell adhesion; cell adhesion; neurite development; positive regulation of epithelial cell proliferation; embryo implantation; positive regulation of cell migration. Disease: Lissencephaly 5

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage