Anti-Kir6.2 Antibody | MyBioSource MBS8200907 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Kir6.2 Antibody

catalog :

MBS8200907

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Kir6.2 expression in HeLa (A), NIH3T3 (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of Kir6.2 staining in human brain cortex formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Kir6.2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8200907

products type :

Antibody

products full name :

Anti-Kir6.2 Antibody

products short name :

[Kir6.2]

products name syn :

[ATP-sensitive inward rectifier potassium channel 11; IKATP; Inward rectifier K(+) channel Kir6.2; Potassium channel, inwardly rectifying subfamily J member 11]

other names :

[ATP-sensitive inward rectifier potassium channel 11 isoform 2; ATP-sensitive inward rectifier potassium channel 11; ATP-sensitive inward rectifier potassium channel 11; potassium channel, inwardly rectifying subfamily J, member 11; IKATP; Inward rectifier K(+) channel Kir6.2; Potassium channel, inwardly rectifying subfamily J member 11]

products gene name :

[KCNJ11]

other gene names :

[KCNJ11; KCNJ11; BIR; HHF2; PHHI; IKATP; TNDM3; KIR6.2; MODY13]

uniprot entry name :

KCJ11_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Porcine, Rabbit

sequence length :

303

specificity :

Recognizes endogenous levels of Kir6.2 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipping: Blue ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000). IHC (1/100 - 1/200). IF (1/100 - 1/500). ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kir6.2. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Kir6.2

ncbi gi num :

261399884

ncbi acc num :

NP_001159762.1

ncbi gb acc num :

NM_001166290.1

uniprot acc num :

Q14654

ncbi pathways :

ABC-family Proteins Mediated Transport Pathway (1269904); ATP Sensitive Potassium Channels Pathway (1268828); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Insulin Secretion Pathway (777534); Integration Of Energy Metabolism Pathway (1270101); Inwardly Rectifying K+ Channels Pathway (1268824); Metabolism Pathway (1269956); Neuronal System Pathway (1268763); Potassium Channels Pathway (1268821); Regulation Of Insulin Secretion Pathway (1270102)

ncbi summary :

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and is found associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive disorder characterized by unregulated insulin secretion. Defects in this gene may also contribute to autosomal dominant non-insulin-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2009]

uniprot summary :

Kir6.2: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation. Defects in KCNJ11 are the cause of familial hyperinsulinemic hypoglycemia type 2 (HHF2); also known as persistent hyperinsulinemic hypoglycemia of infancy (PPHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. Defects in KCNJ11 are a cause of diabetes mellitus permanent neonatal (PNDM). PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy. Defects in KCNJ11 are the cause of transient neonatal diabetes mellitus type 3 (TNDM3). Neonatal diabetes mellitus, defined as insulin-requiring hyperglycemia within the first month of life, is a rare entity. In about half of the neonates, diabetes is transient and resolves at a median age of 3 months, whereas the rest have a permanent form of diabetes. In a significant number of patients with transient neonatal diabetes mellitus, diabetes type 2 appears later in life. The onset and severity of TNDM3 is variable with childhood-onset diabetes, gestational diabetes or adult-onset diabetes described. Defects in KCNJ11 may contribute to non-insulin- dependent diabetes mellitus (NIDDM), also known as diabetes mellitus type 2. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily. Protein type: Membrane protein, integral; Channel, potassium; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 11p15.1. Cellular Component: voltage-gated potassium channel complex; mitochondrion; endoplasmic reticulum; integral to plasma membrane; T-tubule; ATP-sensitive potassium channel complex; nuclear envelope; axolemma; cytosol; cell soma; plasma membrane; endosome; myelin sheath. Molecular Function: protein C-terminus binding; potassium ion binding; voltage-gated potassium channel activity; G-protein activated inward rectifier potassium channel activity; heat shock protein binding; ATP-activated inward rectifier potassium channel activity; ankyrin binding; ATP binding. Biological Process: response to drug; synaptic transmission; regulation of membrane potential; potassium ion import; energy reserve metabolic process; glucose metabolic process; response to testosterone stimulus; regulation of insulin secretion; response to estradiol stimulus; response to ATP; negative regulation of insulin secretion; neurological system process. Disease: Maturity-onset Diabetes Of The Young, Type 13; Hyperinsulinemic Hypoglycemia, Familial, 2; Diabetes Mellitus, Transient Neonatal, 3; Diabetes Mellitus, Permanent Neonatal; Diabetes Mellitus, Noninsulin-dependent

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage