product summary
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company name :
MyBioSource
product type :
antibody
product name :
O-GalNAc Antibody, Clone 9B9: Alkaline Phosphatase
catalog :
MBS808565
quantity :
0.1 mg
price :
505 USD
clonality :
monoclonal
host :
mouse
conjugate :
AP
clone name :
[9B9]
application :
western blot, ELISA, immunocytochemistry, enzyme immunoassay
more info or order :
image
image 1 :
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
image 2 :
Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
product information
catalog number :
MBS808565
products type :
Antibody
products full name :
O-GalNAc Antibody, Clone 9B9: Alkaline Phosphatase
products short name :
[O-GalNAc]
products name syn :
[Mouse Anti-beta-O-Linked N-Acetylgalactosamine Monoclonal IgM; O-GalNAc Antibody: Alkaline Phosphatase; GalNAC Antibody; O-GalNAC Antibody; beta-O-Linked N-Acetylgalactosamine Antibody; O-Linked N-Acetylgalactosamine Antibody; N-Acetylgalactosamine Antibody; 2-(Acetylamino)-2-deoxy-D-galactose Antibody; 2-Acetamido-2-deoxy-D-galactose Antibody; N-Acetylchondrosamine Antibody; 2-Acetamido-2-deoxy-D-galactopyranose Antibody; N-Acetyl-D-galactosamine Antibody; alpha-N-Acetylgalactosamine Antibody; N-Acetylgalactosamine-modified protein Antibody]
products gene name :
[O-GalNAc]
clonality :
Monoclonal
isotype :
IgM
clone :
[9B9]
host :
Mouse
reactivity :
Species Independent
specificity :
Specific for beta-O-Linked N-Acetylgalactosamine and beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
purity :
PEG Purified
concentration :
1 mg/ml
storage stability :
Store at -20°C for 1 year. Avoid freeze/ thaw cycles.
tested application :
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
app notes :
WB: 1:1000). ICC/IF (1:50). ELISA (1:1000). Optimal dilutions for assays should be determined by the user.
image1 heading :
Immunocytochemistry/Immunofluorescence (ICC/IF)
image2 heading :
Western Blot (WB)
other info1 :
Immunogen: Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH). Conjugate: Alkaline Phosphatase. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
other info2 :
Certificate of Analysis: A 1:1000 dilution of MBS808565 was sufficient for detection of O-GlcNAc in 2 ug of GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary antibody.
products categories :
Antibodies; Cell Signaling; Post-translational Modifications; Glycation
size1 :
0.1 mg
price1 :
505 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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