Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B | MyBioSource MBS807323 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B

catalog :

MBS807323

quantity :

0.1 mg

price :

435 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[S396-29]

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.

product information

catalog number :

MBS807323

products type :

Antibody

products full name :

Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B

products short name :

[Nav beta 3]

products name syn :

[Sodium channel subunit beta-3 Antibody; Scn3b Antibody; KIAA1158 Antibody; Nav beta 3 Antibody: APC ; Mouse Anti-Rat NaVbeta3 Monoclonal, Clone S396-29; Anti-Nav beta 3; Nav beta 3 Antibody]

other names :

[sodium channel subunit beta-3; Sodium channel subunit beta-3; sodium channel subunit beta-3; sodium voltage-gated channel beta subunit 3]

products gene name :

[Nav beta 3]

other gene names :

[Scn3b; Scn3b; Scnb3]

uniprot entry name :

SCN3B_RAT

clonality :

Monoclonal

isotype :

IgG2B

clone :

[S396-29]

host :

Mouse

reactivity :

Mouse, Rat

sequence length :

215

specificity :

Detects ~40kDa. Does not cross react with NavBeta1, Navbeta2, or Navbeta3

purity :

Protein G Purified

concentration :

1 mg/ml

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

Western Blot: 1:1000

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Fusion protein amino acids 1-215 (full-length) of rat NavBeta3. Immunogen Species: Rat

other info2 :

COA: A 1:100 dilution was sufficient for detection of NavBeta3 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide. Conjugate: APC . Cellular Localization: Membrane

products categories :

Neuroscience; Ion Channels; Sodium Channels; Voltage-Gated Sodium Channels

products description :

Nav Beta 3 (SCN3B) belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents which are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). Defects in SCN3B are the cause of Brugada syndrome (3).

products references :

1. Genes and mapped phenotypes. (n.d.). Retrieved March 26, 2015, from http://www.ncbi.nlm.nih.gov/gene/?term=Q9jK00 2. Qu Y., et al. (2001) Mol Cell Neurosci. 18(5): 570-580. 3. Ishikawa T., et al. (2013) Circ J. 77(4): 956-967.

ncbi gi num :

20806175

ncbi acc num :

NP_620797.1

ncbi gb acc num :

NM_139097.3

uniprot acc num :

Q9JK00

ncbi mol weight :

Detects ~40kDa.

ncbi summary :

voltage-sensitive sodium channel auxiliary subunit; modulates channel gating with distinct kinetics [RGD, Feb 2006]

uniprot summary :

SCN3B: Modulates channel gating kinetics. Causes unique persistent sodium currents. Inactivates the sodium channel opening more slowly than the subunit beta-1. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN3B are the cause of Brugada syndrome type 7 (BRGDA7). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family. Protein type: Channel, sodium; Membrane protein, integral. Cellular Component: integral to membrane; plasma membrane; voltage-gated sodium channel complex; Z disc. Molecular Function: sodium channel regulator activity. Biological Process: cardiac muscle contraction; membrane depolarization; nervous system development; positive regulation of heart rate; sensory perception of pain; sodium ion transport

size1 :

0.1 mg

price1 :

435 USD

more info or order :

MyBioSource product webpage