NCC Antibody: ATTO 594; Rabbit Anti-Rat NCC Antibody Polyclonal | MyBioSource MBS805451 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NCC Antibody: ATTO 594; Rabbit Anti-Rat NCC Antibody Polyclonal

catalog :

MBS805451

quantity :

0.1 mg

price :

450 USD

clonality :

polyclonal

host :

rabbit

conjugate :

ATTO 594

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000.

product information

catalog number :

MBS805451

products type :

Antibody

products full name :

NCC Antibody: ATTO 594; Rabbit Anti-Rat NCC Antibody Polyclonal

products short name :

[NCC]

products name syn :

[SLC12A3 Antibody, SCYL1 Antibody,CKb10 Antibody, MCP-4 Antibody, MGC17134 Antibody, NCC-1 Antibody, NCC1 Antibody, SCYA13 Antibody, CK-beta-10 Antibody, monocyte chemoattractant protein 4 Antibody, monocyte chemotactic protein 4 Antibody, new CC chemokine 1 Antibody, small inducible cytokine A13 Antibody, small inducible cytokine subfamily A (Cys-Cys) member 13 Antibody, chemokine (C-C) Antibody]

other names :

[solute carrier family 12 member 3 isoform 1; Solute carrier family 12 member 3; solute carrier family 12 member 3; Na-Cl symporter; Na-Cl cotransporter; thiazide-sensitive Na-Cl cotransporter; thiazide-sensitive sodium-chloride cotransporter; NaCl electroneutral thiazide-sensitive cotransporter; solute carrier family 12 (sodium/chloride transporter), member 3; Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter]

other gene names :

[SLC12A3; Slc12a3; NCC; TSC; NCCT; Tsc]

uniprot entry name :

S12A3_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Dog

sequence length :

1030

specificity :

Detects ~160kDa

purity :

Affinity Purified

concentration :

1mg/mL

storage stability :

Store at -20°C; 1 year+ Avoid freeze/thaw cycles. Shipping: Blue Ice or 4°C

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), IEM

app notes :

WB: 1:1000. Optimal dilutions for assays should be determined by the end user.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Conjugate: ATTO 594. Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal. Certificate of Analysis: 1ug/ml of MBS805451 was sufficient for detection of NCC3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-Rabbit IgG: HRP as the secondary antibody. Research Areas: Cancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters. Cellular Localization: Membrane. Sequence References: Gene ID:54300,6559

other info2 :

Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide

products categories :

Ion Channels, Transporters

products description :

Function: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

products references :

1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.

ncbi gi num :

186910315

ncbi acc num :

NP_000330

ncbi gb acc num :

NM_000339.2

uniprot acc num :

P55018

ncbi mol weight :

~160kDa

ncbi pathways :

Cation-coupled Chloride Cotransporters Pathway (119563); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway (119559)

ncbi summary :

This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

NCCT: Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. Interacts with KLHL3. Predominant in kidney. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral. Cellular Component: apical plasma membrane; plasma membrane; integral to membrane; cytosol; vesicle. Molecular Function: sodium ion transmembrane transporter activity; transporter activity; sodium:chloride symporter activity; cation:chloride symporter activity. Biological Process: transport; sodium ion transport; chloride transport

size1 :

0.1 mg

price1 :

450 USD

more info or order :

MyBioSource product webpage