antibody

Rab4 Antibody: ATTO 488

MBS805201

0.1 mL

395 USD

polyclonal

rabbit

ATTO 488

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

Antibody

Rab4 Antibody: ATTO 488

Rab4

Oncogene Rab4; Rab4A; Ras related protein

Ras-related protein Rab-5A; ras-related protein Rab-5A; RAB5A, member RAS oncogene family

RAB5A; RAB5A; RAB5; RAB5

RAB5A_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

201

Rabbit antiserum.

-20 degree C

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

WB: 1:1000-2000 . ICC/IF: 1:150 . Optimal dilutions for assays should be determined by the user.

Conjugate: ATTO 488. Immunogen: C-terminal peptide from human Rab4. Gene ID: 5867. Acession Number: NP_004569.2. Swiss Prot: P02338

Storage Buffer: Rabbit Antiserum. Certificate of Analysis: 0.5ug/ml of MBS805201 was sufficient for detection of Rab4 in 10ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG: HRP as the secondary antibody. Reasearch Area(s): Cell signaling Oraganelle Markers Neuroscience Cell Structure Pre-Synaptic Markers. Cellular Localization: Membrane Cytoplasm

Cell Signaling, Neuroscience, Organelle Markers

Background Info: Detects ~26kDa. Scientific Background: Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4). In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).

1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Cormont M., et al. (1996) Mol Cell Biology. 16: 6879-6886. 6. Ayad N., Hull M., and Mellman I. (1997) EMBO 16: 4497-4507. 7. Van der Sluijs, P. et l. (1992) The EMBO Journal 11(12), 4379-4389. 1. Ballmer-Hofer, K., Andersson, A.E., Ratcliffe, L.E. and Berger, P. (2011). Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output. Blood. 118 (3), 816-826. doi: 10.1182/blood-2011-01-328773.

NP_004569.3

P20339

22,178 Da

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family. Protein type: G protein, monomeric; G protein; Motility/polarity/chemotaxis; G protein, monomeric, Rab. Chromosomal Location of Human Ortholog: 3p24-p22. Cellular Component: zymogen granule membrane; protein complex; dendrite; early endosome; terminal button; phagocytic vesicle; cytosol; lipid raft; actin cytoskeleton; ruffle; synaptic vesicle; recycling endosome; cell soma; perinuclear region of cytoplasm; endocytic vesicle; axon; cytoplasm; melanosome; plasma membrane; endosome membrane; nerve terminal; endosome. Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; endocytosis; regulation of filopodium formation; positive regulation of smooth muscle cell migration; Rab protein signal transduction; blood coagulation; positive regulation of exocytosis

0.1 mL

395 USD