Anti-Rhodopsin Antibody: Clone 4D2 | MyBioSource MBS803218 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Rhodopsin Antibody: Clone 4D2

catalog :

MBS803218

quantity :

0.025 mg

price :

255 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[4D2]

reactivity :

cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2. Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS - inner segment; ONL - outer nuclear layer; OPL - outer plexiform layer; INL - inner nuclear layer; IPL - inner plexiform layer; GCL - ganglion cell layer.

product information

catalog number :

MBS803218

products type :

Antibody

products full name :

Anti-Rhodopsin Antibody: Clone 4D2

products short name :

[Rhodopsin]

products name syn :

[OPN2; opsd; opsin 2; opsin 2 rod pigment; opsin2; RHO; RP4; MGC138309; Retinitis Pigmentosa 4]

other names :

[rhodopsin; Rhodopsin; rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); rhodopsin]

other gene names :

[RHO; RHO]

uniprot entry name :

OPSD_BOVIN

clonality :

Monoclonal

isotype :

IgG1

clone :

[4D2]

host :

Mouse

reactivity :

Mm, Fs, Av,Am, Shk

sequence length :

348

specificity :

Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.

form :

Protein G Purified

concentration :

1mg/mL

storage stability :

-20°C; 1 year + Avoid freeze/thaw cycle. Shipping: Blue Ice or 4°C.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunprecipitation (IP), ELISA (EIA)

app notes :

WB: 1:1000. Optimal dilutions for assays should be determined by the user.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Bovine Rhodosin. Certificate of Analysis: 1 ug/mL of MBS803218 was sufficient for detection of rhodopsin in 10ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Research Areas: Neuroscience, Neutransmitter Receptors, Rhodopsin, Cell Signaling. Cellular Localization: Membrane

other info2 :

Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide

products categories :

GTPase

products description :

Background Info: Detects ~40kDa band corresponding to the molecular weight of Rhodopsin. Binds specifically to the N-terminus of Rhodopsin. Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

products references :

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567. 1. Cheng, C.L., Djajadi, H. and Molday, R.S. (2012). Cell-Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy. Methods in Molecular Biology. 935, 185-199. doi: 10.1007/978-1-62703-080-9_12

ncbi gi num :

62460472

ncbi acc num :

NP_001014890.1

ncbi gb acc num :

NM_001014890.1

uniprot acc num :

P02699

ncbi pathways :

Activation Of The Phototransduction Cascade Pathway (1008637); Class A/1 (Rhodopsin-like Receptors) Pathway (1008554); Disease Pathway (942846); Diseases Associated With Visual Transduction Pathway (1008713); G Alpha (i) Signalling Events Pathway (1008584); GPCR Downstream Signaling Pathway (1008582); GPCR Ligand Binding Pathway (1008553); GPCRs, Class A Rhodopsin-like Pathway (920903); Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway (942909); Integrin-mediated Cell Adhesion Pathway (198555)

uniprot summary :

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal ().

size1 :

0.025 mg

price1 :

255 USD

more info or order :

MyBioSource product webpage