Anti-KDEL Receptor Antibody: Clone KR-10 | MyBioSource MBS802828 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-KDEL Receptor Antibody: Clone KR-10

catalog :

MBS802828

quantity :

0.1 mg

price :

415 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[KR-10]

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.

image 2 :

Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

product information

catalog number :

MBS802828

products type :

Antibody

products full name :

Anti-KDEL Receptor Antibody: Clone KR-10

products short name :

[KDEL]

products name syn :

[ERD2; ERD2.1; ERD21; HDEL; KDEL; KDEL R1; KDELR1; PM23]

other names :

[ER lumen protein-retaining receptor 1; ER lumen protein-retaining receptor 1; ER lumen protein-retaining receptor 1; KDEL receptor 1; ER lumen protein retaining receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1]

other gene names :

[Kdelr1; Kdelr1; Erd2.1; AW215843; 8030486F04Rik; KDEL receptor 1]

uniprot entry name :

ERD21_MOUSE

clonality :

Monoclonal

isotype :

IgG1

clone :

[KR-10]

host :

Mouse

reactivity :

Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, Xenopus

sequence length :

212

form :

Protein G Purified

concentration :

1mg/mL

storage stability :

-20 degree C; 1 year +. Avoid freeze/thaw cycles. Blue Ice or 4 degree C.

tested application :

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

1:1000 dilution was sufficient for detection by colorimetric analysis

image1 heading :

Immunocytochemistry/Immunofluorescence (ICC/IF)

image2 heading :

Western Blot (WB)

other info1 :

Conjugate: FITC. Immunogen: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH.

other info2 :

Storage Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide. COA: 1ug/ml was sufficient for detection of KDEL receptor in 20 ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG: AP as the secondary. Research Area(s): Cell signaling, Oraganelle Markers, Trafficking, Chaperones. Cellular Localization: Endoplasmic reticulum.

products categories :

Chaperones, Organelle Markers, Trafficking

products description :

Background Info: Detects an ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots. Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).

products references :

1. Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737. 2. Forthoffer N., et al. (2002) J Bioenerg Biomemb 34(3): 209-219. 3. Aoe T., et al. (1997) EMBO J. 16: 7305-7316. 4. Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328. 5. Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163. 6. Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from http://cellbio.utmb.edu/cellbio/rer1.htm

ncbi gi num :

19527170

ncbi acc num :

NP_598711.1

ncbi gb acc num :

NM_133950.2

uniprot acc num :

Q99JH8

ncbi mol weight :

~25kDa

uniprot summary :

KDELR1: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L. Belongs to the ERD2 family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Endoplasmic reticulum. Cellular Component: Golgi apparatus; cis-Golgi network; membrane; endoplasmic reticulum; integral to membrane; ER-Golgi intermediate compartment; cytoplasmic vesicle. Molecular Function: ER retention sequence binding. Biological Process: vesicle-mediated transport; protein transport; transport; protein retention in ER

size1 :

0.1 mg

price1 :

415 USD

more info or order :

MyBioSource product webpage