antibody

DNA/RNA Damage Antibody, 15A3; Anti-DNA/RNA Damage Antibody

MBS802475

0.025 mg

230 USD

monoclonal

mouse

nonconjugated

[15A3]

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, dot blot

Antibody

DNA/RNA Damage Antibody, 15A3; Anti-DNA/RNA Damage Antibody

[DNA/RNA Damage]

[8-Hydroxy Guanine; 8-OH-dG; 8OHG; 80G; 80G, 8 hydroxyguanine; 8 hydroxy 2' deoxyguanosine; 8 hydroxyguanosine; 8 OHG; 8-OHG; 8OHdG]

Monoclonal

IgG2b

[15A3]

Mouse

All

Recognizes markers of oxidative damage to DNA (8-hyroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine

Protein G Purified

1mg/mL

Store at -20°C for 1 year+. Avoid freeze/thaw cycles. Shipping: Blue Ice

Immunohistochemitsry (IHC), Immunocytochemistry (ICC), Immunofluroescence (IF), ELISA, Dot Blot (DB), Immunoprecipitation (IP), Flow Cytometry (FCM), Flow Assay (FA)

IHC: (1:1000); optimal dilutions for assays should be determined by end user

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: Ischemic fresh brain tissue. Species: Rat. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Alexa Fluor 546 Goat Anti-mouse (Red) at 1:500 for 1 hour at RT. Localization: Cerebral Cortex. Courtesy of: Dr. Yi Yang, U. New Mexico.

Immunogen: 8-hydroxy-guanosine-BSA and -casein conjugates

Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide. Research Areas: Cancer; Oxidative Stress; Cell Signaling; Post-Transitional Modifications; Oxidation; Epigenetics and Nuclear Signaling; DNA/RNA; DNA Damage and Repair

Oxidative Stress, Post-translational Modifications

Scientific Background: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage (1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).

1. Kim H.W., Murakami A., Williams M.V., and Ohigashi H. (2003) Carcinogenesis 24(2): 235-241. 2. Pilger A. and Rudiger H.W. (2006) Int Arch Occup Environ Health. 80(1): 1-15. 3. Malins D.C. and Haimanot R. (1991) Cancer Res. 51(19): 5430-5432. 4. Kvam E. and Tyrrell R.M. (1997) Carcinogenesis 18(11): 2281-2283. 5. Kowluru R.A., Atasi L., and Ho Y.S. (2006) Invest Ophthalmol Vis Sci 47(4): 1594-9. 6. Bowers R. et al. (2004) Am J Respir Crit Care Med. 169(6): 764-9. 7. Cui J., Holmes E.H., Greene T.G., and Liu P.K. (2000) Faseb J. 14(7): 955-67.

0.025 mg

230 USD