antibody

GRP78 Antibody

MBS801077

0.1 mg

380 USD

monoclonal

mouse

nonconjugated

[3C5-1A4]

human, mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

GRP78 Antibody

[GRP78]

[GRP78 Antibody, 3C5-1A4 ; 78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+F6inding protein grp78 Antibody, Endoplasmic reticulum lumenal Al1tibody, Ca2+ binding protein grp78 Antibody, FU26106 Antibody, Glucose Regulated Protein 78kDa Antibody, GRP 78 Antibody, GRP-78 Antibody, GRP78_HUMAN Antibody, Heat shock 70 kDa protein 5 Antibody, Heat Shock 70kDa Protein 5 Antibody, HSCe70 Antibody, HSPA 5 Antibody, HSPA5 Antibody, Immunoglobulin Heavy Chain Binding Protein Antibody, Immunoglobulin heavy chain-binding protein Antibody, mBiP Antibody, MIF2 Antibody, Sez7 Antibody]

[78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; GRP 78; GRP-78; heat shock 70kD protein 5; heat shock 70 kDa protein 5; steroidogenesis-activator polypeptide; immunoglobulin heavy chain-binding protein; heat shock 70kDa protein 5 (glucose-regulated protein); heat shock protein 5; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; Steroidogenesis-activator polypeptide]

[Hspa5; Hspa5; BIP; GRP78; Grp78; GRP-78; BiP]

GRP78_RAT

Monoclonal

IgG1 Kappa

[3C5-1A4]

Mouse

Human, Mouse, Rat

654

Detects ~78kDa

Protein G Purified

1mg/mL

Store at -20°C; 1 year +; Avoid freeze/thaw cycle

Western Blot (WB), ELISA (EIA)

WB (1:1000); optimal dilutions for assays should be determined by the user.

Western Blot (WB)

Certificate of Analysis: 1 ug/mL was sufficient for detection of GRP78 in 20ug of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Immunogen: Full-length recombinant rat GRP78

Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. Research Area: Cancer; Heat Shock; Cell Signaling; Trafficking; Chaperones; Organelle Markers. Cellular Localization: Endoplasmic reticulum lumen; Melanosome

Chaperones, Heat Shock, Organelle Markers, Trafficking

Background Info: Detects a protein with a mass of ~78kDa corresponding to GRP78. Scientific Background: GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

1. Cho, S. et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19:4333- 4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.

25742763

NP_037215.1

NM_013083.2

P06761

ATF6-alpha Activates Chaperone Genes Pathway (1009784); ATF6-alpha Activates Chaperones Pathway (1009783); Antigen Processing And Presentation Pathway (83466); Antigen Processing And Presentation Pathway (485); Hemostasis Pathway (1010891); MAPK Signaling Pathway (198496); Metabolism Of Proteins Pathway (1009706); Platelet Activation, Signaling And Aggregation Pathway (1010901); Platelet Degranulation Pathway (1010913); Prion Diseases Pathway (101113)

secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006]

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Protein type: Chaperone; Heat shock protein. Cellular Component: signalosome; endoplasmic reticulum membrane; smooth endoplasmic reticulum; focal adhesion; cell surface; mitochondrion; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment; membrane; melanosome; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus; myelin sheath. Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; unfolded protein binding; ribosome binding; misfolded protein binding; glycoprotein binding; ATP binding. Biological Process: cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; cerebellum structural organization; substantia nigra development; proteolysis involved in cellular protein catabolic process; positive regulation of protein ubiquitination; cerebellar Purkinje cell layer development; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; positive regulation of cell migration; negative regulation of apoptosis

0.1 mg

380 USD