HIF2 alpha Antibody: APC | MyBioSource MBS800144 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HIF2 alpha Antibody: APC

catalog :

MBS800144

quantity :

0.1 mg

price :

405 USD

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

[EP190B]

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HIF2 alpha protein using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Cells CoCl treated.

image 2 :

Immunohistochemistry analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, dermis positive and muscle positive.

product information

catalog number :

MBS800144

products type :

Antibody

products full name :

HIF2 alpha Antibody: APC

products short name :

[HIF2 alpha]

products name syn :

[ECYT4; EPAS1; HIF2alpha; HIF1 Alpha like factor; HLF; Hypoxia inducible factor 2 alpha; MOP2; PASD2]

other names :

[endothelial PAS domain-containing protein 1; Endothelial PAS domain-containing protein 1; endothelial PAS domain-containing protein 1; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-1alpha-like factor; HIF-1-alpha-like factor; member of PAS protein 2; PAS domain-containing protein 2; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2]

other gene names :

[EPAS1; EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73; BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha]

uniprot entry name :

EPAS1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[EP190B]

host :

Mouse

reactivity :

Human, Mouse

sequence length :

870

specificity :

~100kDa. Specific for HIF2 Alpha

form :

Protein G Purified

concentration :

1mg/mL

storage stability :

Store at -20°C; 1 year+. Avoid freeze/thaw cycles. Shipping Temperature: Blue Ice or 4°C

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

WB (1:500). ICC/IF (1:100). Optimal dilutions for assays should be determined by the user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: GST-human EPAS-1 (HIF2alpha) amino acids 535-631 fusion protein. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. Certificate of Analysis: 1 ug/ml of SMC-185 was sufficient for detection of HIF2-alpha in 20 ug of CoCl2-induced Hela cell lystae by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Research Areas: Cancer Oxidative stress Cell Signaling Epigenetics. Cellular Localization: Nucleus.

products categories :

Cell Signaling, Oxidative Stress

products description :

Background Info: Does not cross react with HIF1Alpha. Scientific Background: Members of the hypoxia-inducible factor (HIF) family of transcription factors regulate the cellular response to hypoxia (1). HIF2-Alpha is involved in catecholamine homeostasis, vascular remodeling, physiological angiogenesis and adipogenesis. It is overexpressed in many cancerous tissues, but its exact role in tumour progression remains to be clarified (2). Studies suggest that in the case of non-small cell lung cancer, HIF2-Alpha actually is a promoter of tumor growth and progression in a solid tumor (1). Other data suggests that HIF2-Alpha is an important regulator of innate immunity, and therefore may be useful in the therapeutic target for treating inflammatory disorders and cancer (3).

products references :

1. Kim W.Y., et al. (2009) J Clin Invest. 119(8): 2160-2170. 2. Favier J., Lapointe S., Maliba R. and Sirois M.G. (2007) BMC Cancer. 7:139. 3. Imtiyaz H.Z., et al. (2010) J Clin Invest. 120(8): 2699-2714.

ncbi gi num :

40254439

ncbi acc num :

NP_001421

ncbi gb acc num :

NM_001430.4

uniprot acc num :

Q99814

ncbi mol weight :

96,459 Da

ncbi pathways :

Adipogenesis Pathway (198832); Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Developmental Biology Pathway (477129); HIF-2-alpha Transcription Factor Network Pathway (137956); Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (645261); Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (645262); Pathways In Cancer (83105); Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway (645260); Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway (645263)

ncbi summary :

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

uniprot summary :

HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5 -[AG]CGTG-3 within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 2p21-p16. Cellular Component: nucleoplasm; transcription factor complex; cytosol. Molecular Function: histone acetyltransferase binding; signal transducer activity; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor binding. Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; myoblast cell fate commitment; embryonic placenta development; regulation of heart rate; somatic stem cell maintenance; cell maturation; norepinephrine metabolic process; signal transduction; iron ion homeostasis; visual perception; response to hypoxia; erythrocyte differentiation; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; surfactant homeostasis; lung development. Disease: Erythrocytosis, Familial, 4

size1 :

0.1 mg

price1 :

405 USD

more info or order :

MyBioSource product webpage