Mouse Anti-Rat GluN2B/NR2B Monoclonal IgG2b | MyBioSource MBS800075 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Rat GluN2B/NR2B Monoclonal IgG2b

catalog :

MBS800075

quantity :

0.1 mg

price :

390 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

S59-36

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; mouse; loading ...; fig 2a	Lu F, Shao G, Wang Y, Guan S, Burlingame A, Liu X, et al. Hypoxia-ischemia modifies postsynaptic GluN2B-containing NMDA receptor complexes in the neonatal mouse brain. Exp Neurol. 2018;299:65-74 pubmed publisher

product information

catalog number :

MBS800075

products type :

Antibody

products full name :

Mouse Anti-Rat GluN2B/NR2B Monoclonal IgG2b

products short name :

NR2B

products name syn :

NMDAR2B; hNR3; MGC142180; glutamate receptor subunit epsilon-2; NR3

other names :

glutamate receptor ionotropic, NMDA 2B; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B

other gene names :

Grin2b; Grin2b; GluN2B; GluN2B; NMDAR2B; NR2B

uniprot entry name :

NMDE2_RAT

clonality :

Monoclonal

isotype :

IgG2b

clone :

S59-36

host :

Rat

reactivity :

Human, Rat, Mouse

sequence length :

1482

specificity :

Detects ~166kDa. No cross-reactivity against NR2A

form :

Protein G Purified

concentration :

1mg/mL

storage stability :

-20 degree C. Avoid freeze/thaw cycles. Shipping Temperature: 4 degree C

tested application :

Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB: (1:1000), IHC: (1:1000), ICC/IF (1:1000). Optimal dilutions for assays should be determined by the user.

other info1 :

Certificate of Analysis: 1ug/mL was sufficient for detection of NR2B in 10ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Immunogen: Fusion protein amino acids 20-127 (extracellular N-terminus) of rat NR2B.

other info2 :

Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. Cellular Localization: Cell membrane, Cell Junction, synapse, postsynaptic cell membrane. Research Areas: Neuroscience, Ion Channels, Neurotransmitter Receptors, Glutamate Receptors, NMDA Receptors, Neurodegeneration. Conjugates: Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

products categories :

Ion Channels, Neuroscience

products description :

Background Info: Detects ~166kDa. No cross-reactivity against NR2A. Scientific Background: NR2B containing receptors have been implicated in synaptic plasticity, memory formation and pain modulation (3). Studies suggest that the NR2B subunit of glutamate receptors may be potential targets for relieving pain; NR2B may be a probable target for anti-nociceptive drugs, and may also be useful as analgesics (4).

products references :

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Zhuo M. (2009) Mol Brain. 2(1): 4 4. Gurwitz D, and Weizman A. (2002) Drug Discovery Today, 7(7): 403-406.

ncbi gi num :

6980984

ncbi acc num :

NP_036706.1

ncbi gb acc num :

NM_012574.1

uniprot acc num :

Q00960

ncbi pathways :

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Circadian Entrainment Pathway (698787); Circadian Entrainment Pathway (699872)

ncbi summary :

subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

uniprot summary :

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Protein type: Membrane protein, multi-pass; Channel, calcium; Channel, ligand-gated; Membrane protein, integral. Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; Z disc; N-methyl-D-aspartate selective glutamate receptor complex. Molecular Function: neurotransmitter binding; interleukin-1 receptor binding; zinc ion binding; calcium channel activity; beta-catenin binding; drug binding; ionotropic glutamate receptor binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding; cell adhesion molecule binding; cation channel activity; receptor binding; D2 dopamine receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; behavioral fear response; startle response; response to toxin; rhythmic process; positive regulation of glutamate secretion; regulation of synaptic plasticity; response to other organism; receptor clustering; response to organic cyclic substance; positive regulation of synaptic transmission; response to carbohydrate stimulus; synaptic transmission; behavioral response to pain; response to magnesium ion; learning and/or memory; calcium ion transport; response to methylmercury; response to electrical stimulus; associative learning; cation transport; cellular response to magnesium starvation; synaptic transmission, glutamatergic; regulation of action potential; in utero embryonic development; hippocampus development; response to amphetamine; learning; response to cocaine; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; sensory organ development; regulation of postsynaptic membrane potential; response to ethanol; suckling behavior; long-term memory; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; response to cytokine stimulus; regulation of MAPKKK cascade; cerebral cortex development; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; response to amine stimulus

size1 :

0.1 mg

price1 :

390 USD

more info or order :

MyBioSource product webpage