catalog number :
MBS775677
products type :
ELISA Kit
products full name :
Mouse Anti-Double Stranded DNA IgG (DSDNA IgG) ELISA Kit
products short name :
[Anti-Double Stranded DNA IgG (DSDNA IgG)]
products gene name :
[DSDNA]
specificity :
This assay has high sensitivity and excellent specificity for detection of DRP4. No significant cross-reactivity or interference between DRP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DRP4 and all the analogues, therefore, cross reaction may still exist in some cases.
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.
products description :
Intended Uses: This DRP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DRP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: DRP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DRP4 antibody and an DRP4-HRP conjugate. The assay sample and buffer are incubated together with DRP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRP4 concentration since DRP4 from samples and DRP4-HRP conjugate compete for the anti-DRP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRP4 from the sample, fewer sites are left to bind DRP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRP4 concentration in each sample is interpolated from this standard curve.
size4 :
10x96-Strip-Wells