product summary
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company name :
MyBioSource
product type :
antibody
product name :
GADD34 Rabbit Polyclonal
catalog :
MBS769525
quantity :
0.1 mg
price :
290 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
fruit fly
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay
more info or order :
image
image 1 :
Immunohistochemistry of paraffin-embedded human pancreas cancer using MBS769525 (GADD34 antibody) at dilution of 1:100
image 2 :
Immunofluorescent analysis of PC-3 cells using MBS769525 ( GADD34 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
image 3 :
K-562 cells were subjected to SDS PAGE followed by western blot with MBS769525 (GADD34 antibody) at dilution of 1:600
product information
catalog number :
MBS769525
products type :
Antibody
products full name :
GADD34 Rabbit Polyclonal
products short name :
[GADD34]
products name syn :
[GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A]
other names :
[Gadd34; CG3825 gene product from transcript CG3825-RA]
products gene name :
[GADD34]
other gene names :
[Gadd34; CG3825; dGADD34; Dm-GADD34; DmelCG3825; dPPP1R15; gadd34; GADD34]
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human, Mouse, Rat; other species are not tested.
sequence length :
317
specificity :
Please decide the specificity by homology
purity :
>=95% as determined by SDS-PAGE. Immunogen Affinity Purified
form :
Liquid
storage stability :
Store at -20°C for 24 months (Avoid repeated freeze / thaw cycles.)
tested application :
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
app notes :
WB 1:200-1 :1000. IHC 1:20-1:200. IF 1:20-1:200
image1 heading :
Immunohistochemistry
image2 heading :
Immunofluorescent
image3 heading :
SDS-PAGE
other info1 :
Immunogen: Protein phosphatase 1, regulatory (inhibitor) subunit 15A. Calculated MW: 100 kDa
other info2 :
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3
products description :
GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 appears to be about 100 kDa which was confirmed in GADD34 KO mice and drug treated cells, despite the predicated MW of 73 kDa, and Proteintech's GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.
ncbi gi num :
19922868
ncbi acc num :
NP_611863.1
ncbi gb acc num :
NM_138019.3
uniprot acc num :
O75807
size1 :
0.1 mg
price1 :
290 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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