anti- EPOR antibody | MyBioSource MBS769048 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

anti- EPOR antibody

catalog :

MBS769048

quantity :

0.1 mg

price :

320 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunoprecipitation, flow cytometry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using MBS769048 ( EPOR Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

image 2 :

1X10^6 HeLa cells were stained with 0.2ug EPOR antibody (MBS769048, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

image 3 :

IP Result of anti-EPOR (IP: MBS769048, 4ug; Detection: MBS769048 1:500) with HepG2 cells lysate 1920ug.

product information

catalog number :

MBS769048

products type :

Antibody

products full name :

anti- EPOR antibody

products short name :

[EPOR]

products name syn :

[EPO R, EPOR, erythropoietin receptor]

other names :

[erythropoietin receptor; Erythropoietin receptor; erythropoietin receptor; erythropoietin receptor]

products gene name :

[EPOR]

other gene names :

[EPOR; EPOR; EPO-R; EPO-R]

uniprot entry name :

EPOR_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse. Other species are not tested.

sequence length :

508

specificity :

Please decide the specificity by homology

purity :

> 95% as determined by SDS-PAGE; Immunogen affinity purified

form :

Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3

storage stability :

Store -20°C for 24 months (Avoid repeated freeze/thaw cycles.)

tested application :

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC)

app notes :

WB : 1:500-1:5000. IP : 1:200-1:2000. IF : 1:50-1:500

image1 heading :

Immunofluorescent

image2 heading :

Flow Cytometry

image3 heading :

image4 heading :

SDS-PAGE

image4 description :

HepG2 cells were subjected to SDS PAGE followed by western blot with MBS769048 ( EPOR Antibody) at dilution of 1:300

other info1 :

Immunogen: erythropoietin receptor

products description :

Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

ncbi gi num :

4503591

ncbi acc num :

NP_000112.1

ncbi gb acc num :

NM_000121.3

uniprot acc num :

P19235

ncbi mol weight :

769048

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198882); EPO Signaling Pathway (138051); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Kit Receptor Signaling Pathway (198826); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

uniprot summary :

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling. Protein type: Receptor, cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: cytosol; external side of plasma membrane; extracellular region; integral to plasma membrane. Molecular Function: erythropoietin receptor activity; identical protein binding; protein binding. Biological Process: brain development; decidualization; elevation of cytosolic calcium ion concentration; heart development; negative regulation of neuron apoptosis; positive regulation of cell proliferation; signal transduction. Disease: Erythrocytosis, Familial, 1

size1 :

0.1 mg

price1 :

320 USD

more info or order :

MyBioSource product webpage