DAB2 Rabbit Polyclonal | MyBioSource MBS768460 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

DAB2 Rabbit Polyclonal

catalog :

MBS768460

quantity :

0.1 mg

price :

265 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunofluorescent analysis of HepG2 cells, using DAB2 antibody MBS768460 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

image 2 :

HeLa cells were subjected to SDS PAGE followed by western blot with MBS768460 (DAB2 antibody) at dilution of 1:1000

product information

catalog number :

MBS768460

products type :

Antibody

products full name :

DAB2 Rabbit Polyclonal

products short name :

[DAB2]

products name syn :

[DAB2, Disabled homolog 2, DOC 2, DOC2, FLJ26626]

other names :

[disabled homolog 2 isoform 2; Disabled homolog 2; disabled homolog 2; DAB2, clathrin adaptor protein; Adaptor molecule disabled-2; Differentially expressed in ovarian carcinoma 2; DOC-2; Differentially-expressed protein 2]

products gene name :

[DAB2]

other gene names :

[DAB2; DAB2; DOC2; DOC-2; DOC2; DOC-2]

uniprot entry name :

DAB2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

749

specificity :

Human, Mouse ,Rat; other species are not tested. Please decide the specificity by homology

purity :

Purification: Immunogen affinity purified. Purity: > or =95% as determined by SDS-PAGE

form :

Liquid

storage stability :

Store at -20 degree C for 24 months (Avoid repeated freeze/thaw cycles.)

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

app notes :

WB : 1:500-1:5000. IP : 1:1000-1:10000. IF : 1:20-1:200

image1 heading :

Immunofluorescent

image2 heading :

SDS-PAGE

other info1 :

Immunogen: Disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)

other info2 :

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3

products description :

Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR.

ncbi gi num :

349585060

ncbi acc num :

NP_001231800.1

ncbi gb acc num :

NM_001244871.1

uniprot acc num :

P98082

ncbi mol weight :

96 kDa

ncbi pathways :

Endocytosis Pathway (102279); Endocytosis Pathway (102181); Formation Of Annular Gap Junctions Pathway (1269892); Gap Junction Degradation Pathway (1269891); Gap Junction Trafficking Pathway (1269885); Gap Junction Trafficking And Regulation Pathway (1269884); Membrane Trafficking Pathway (1269877); TGF-beta Receptor Signaling Pathway (198774); TGF-beta Receptor Signaling Pathway (138024); Vesicle-mediated Transport Pathway (1269876)

ncbi summary :

This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

DAB2: an adaptor protein that interacts with Grb2, myosin VI, SMAD2/3, DIP1/2, Dvl-3, the integrin betasubunit, and c-Src. Negatively regulates canonical Wnt signaling by stabilizing the beta-catenin degradation complex. A component of the CSF-1 signal transduction pathway. Phosphorylated DAB2 inhibits adhesion and integrin signaling. Two alternatively spliced isoforms are described. Protein type: Tumor suppressor; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 5p13.1. Cellular Component: clathrin coated vesicle membrane; clathrin-coated vesicle; coated pit; focal adhesion; intracellular membrane-bound organelle; lysosomal membrane; nucleolus; plasma membrane. Molecular Function: protein binding; protein C-terminus binding; SMAD binding. Biological Process: apoptosis; cell proliferation; integrin-mediated signaling pathway; leading edge cell differentiation; multicellular organismal development; negative regulation of apoptosis; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; positive regulation of cell migration; positive regulation of endocytosis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; protein transport; receptor-mediated endocytosis; Wnt receptor signaling pathway

size1 :

0.1 mg

price1 :

265 USD

more info or order :

MyBioSource product webpage