CSPG4, NG2 Rabbit Polyclonal | MyBioSource MBS768257 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CSPG4, NG2 Rabbit Polyclonal

catalog :

MBS768257

quantity :

0.1 mg

price :

265 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MBS768257 (CSPG4, NG2 Antibody)at dilution of 1:200 heat mediated antigen retrieved with Tris- EDTA buffer(pH9)

image 2 :

A375 cell were subjected to SDS PAGE followed by western blot MBS768257 (CSPG4, NG2 antibody) at dilution of 1:300

product information

catalog number :

MBS768257

products type :

Antibody

products full name :

CSPG4, NG2 Rabbit Polyclonal

products short name :

[CSPG4, NG2]

other names :

[chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan]

products gene name :

[CSPG4, NG2]

other gene names :

[CSPG4; CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG; MCSP]

uniprot entry name :

CSPG4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat, other species are not tested.

sequence length :

2322

specificity :

Please decide specifity by homology.

purity :

>=95% as determined by SDS-PAGE. Immunogen Affinity Purified

form :

Liquid

storage stability :

-20°C for 24 months (Avoid repeated freeze / thaw cycles.)

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500-1:5000. IHC: 1:50-1:500

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot(WB)

other info1 :

Immunogen: Chondroitin sulfate proteoglycan 4. Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3

other info2 :

Calculated MW: 245 kDa

products description :

CSPG4, also named as HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16 and NG2, is a proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. CSPG4 may inhibit neurite outgrowth and growth cone collapse during axon regeneration. It is cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. CSPG4 may regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. It modulates the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin.

ncbi gi num :

47419930

ncbi acc num :

NP_001888.2

ncbi gb acc num :

NM_001897.4

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (1269981); CS/DS Degradation Pathway (1269987); Chondroitin Sulfate Biosynthesis Pathway (1269985); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1269984); Defective B3GALT6 Causes EDSP2 And SEMDJL1 Pathway (1309217); Defective B3GAT3 Causes JDSSDHD Pathway (1269015); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1269014); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1269018); Defective CHST3 Causes SEDCJD Pathway (1269017); Defective CHSY1 Causes TPBS Pathway (1269016)

ncbi summary :

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

uniprot summary :

NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 15q24.2. Cellular Component: apical plasma membrane; cell surface; extracellular region; focal adhesion; Golgi lumen; integral to plasma membrane; lysosomal lumen. Molecular Function: protein kinase binding; signal transducer activity. Biological Process: activation of MAPK activity; angiogenesis; cell migration; cell proliferation; chondroitin sulfate biosynthetic process; chondroitin sulfate catabolic process; dermatan sulfate biosynthetic process; glial cell migration; glycosaminoglycan metabolic process; positive regulation of peptidyl-tyrosine phosphorylation; tissue remodeling; transmembrane receptor protein tyrosine kinase signaling pathway

size1 :

0.1 mg

price1 :

265 USD

more info or order :

MyBioSource product webpage