antibody

Clusterin Rabbit Polyclonal

MBS768013

0.1 mg

265 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Clusterin Rabbit Polyclonal

[Clusterin]

[AAG4, Apo J, APOJ. ApoJalpha, ApoJbeta, Apolipoprotein J, eLI, CLU, clustcrin. Con1plen1ent cytolysis inhibitor, Ku70 binding protein 1, KUB 1, NAlINA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2]

[clusterin preproprotein; Clusterin; clusterin; clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J]

[CLU; CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; APOJ; CLI; KUB1; Apo-J; CLI; TRPM-2]

CLUS_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse

Please decide the specificity by homology.

>=95% as determined by SDS-PAGE. Immunogen Affinity Purified

Liquid

-20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500-1:2000. IHC: 1:50-1:200

Immunohistochemistry

SDS-PAGE

Immunogen: clusterin

Background: Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into alpha and beta subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

355594753

NP_001822.3

NM_001831.3

P10909

40 kDa

Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1269241); Hemostasis Pathway (1269340); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Platelet Activation, Signaling And Aggregation Pathway (1269350); Platelet Degranulation Pathway (1269367); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1269365); Terminal Pathway Of Complement (1269249)

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]

CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Secreted, signal peptide; Secreted; Mitochondrial. Chromosomal Location of Human Ortholog: 8p21-p12. Cellular Component: cell surface; cytoplasm; cytosol; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; growth cone; intracellular; mitochondrial membrane; mitochondrion; nucleus; perinuclear region of cytoplasm; protein complex. Molecular Function: ATPase activity; chaperone binding; misfolded protein binding; protein binding; protein N-terminus binding; ubiquitin protein ligase binding. Biological Process: activation of NF-kappaB transcription factor; aging; cell morphogenesis; chaperone-mediated protein complex assembly; complement activation; complement activation, classical pathway; endocrine pancreas development; innate immune response; lipid metabolic process; microglial cell activation; myelin maintenance in the central nervous system; negative regulation of protein homooligomerization; neurite morphogenesis; platelet degranulation; positive regulation of apoptosis; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of nitric oxide biosynthetic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of tumor necrosis factor production; protein import; protein stabilization; release of cytochrome c from mitochondria; response to light stimulus; response to misfolded protein; response to virus; response to wounding; reverse cholesterol transport

0.1 mg

265 USD