catalog number :
MBS767104
products full name :
beta actin Mouse Monoclonal
products short name :
[beta actin]
products name syn :
[ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, beta actin]
other names :
[Beta-actin]
reactivity :
Human, Mouse, Rat; other species are not tested. Please decide the specific by homology
purity :
Purification: Protein A+G purification. Purity: > = 95% as determined by SDS-PAGE
storage stability :
Store -20°C for 24 months (Avoid repeated freeze / thaw cycles.)
tested application :
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS),
app notes :
WB:1:2000-1:20000. IHC:1:50-1:500. IF:1:20-1:200
image1 heading :
Immunohistochemistry
image2 heading :
Immunofluorescent
image3 heading :
SDS-PAGE
other info1 :
Immunogen: Actin, beta
other info2 :
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3
products description :
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
ncbi acc num :
SAY41341.1