ATP5C1 Mouse Monoclonal | MyBioSource MBS766937 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ATP5C1 Mouse Monoclonal

catalog :

MBS766937

quantity :

0.1 mg

price :

265 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using MBS766937 (ATP5C1 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9)

image 2 :

Human heart tissue were subjected to SDS-PAGE followed by western blot with MBS766937 (ATP5C1 Antibody) at dilution of 1:1000

product information

catalog number :

MBS766937

products type :

Antibody

products full name :

ATP5C1 Mouse Monoclonal

products short name :

[ATP5C1]

products name syn :

[ATP5C, ATP5C1, ATP5CL1, F ATPase gamma subunit]

other names :

[ATP5C1; ATP synthase subunit gamma, mitochondrial; ATP synthase subunit gamma, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1; F-ATPase gamma subunit]

products gene name :

[ATP5C1]

other gene names :

[ATP5C1; ATP5C1; ATP5C; ATP5CL1; ATP5C; ATP5CL1]

uniprot entry name :

ATPG_HUMAN

clonality :

Monoclonal

isotype :

IgG1

host :

Mouse

reactivity :

Human, other species are not tested.

sequence length :

298

specificity :

Please decide specifity by homology.

purity :

>=95% as determined by SDS-PAGE. Protein A+G purification

form :

Liquid

storage stability :

Store at -20°C for 24 months (Avoid repeated freeze / thaw cycles.)

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Western Blot (WB) 1:500-1:2000. Immunohistochemistry (IHC) 1:100-1:500

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Testing Data (TD)

other info1 :

Immunogen: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1. Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Calculated MW: 33 kDa

products description :

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1)-containing the extramembraneous catalytic core, and F(0)-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

ncbi gi num :

48146923

ncbi acc num :

CAG33684.1

uniprot acc num :

Q6I9V2

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Electron Transport Chain Pathway (198860); F-type ATPase, Eukaryotes Pathway (522535); F-type ATPase, Eukaryotes Pathway (890450); Formation Of ATP By Chemiosmotic Coupling Pathway (1270129); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Metabolic Pathways (132956); Metabolism Pathway (1269956)

ncbi summary :

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq, Jul 2008]

uniprot summary :

ATP5C: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase gamma chain family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.6.3.14; Mitochondrial; Hydrolase; Energy Metabolism - oxidative phosphorylation. Chromosomal Location of Human Ortholog: 10p15.1. Cellular Component: membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex; mitochondrion; myelin sheath. Molecular Function: ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanism; transmembrane transporter activity. Biological Process: ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport; oxidative phosphorylation

size1 :

0.1 mg

price1 :

265 USD

more info or order :

MyBioSource product webpage