ACAT1 Rabbit Polyclonal | MyBioSource MBS766304 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ACAT1 Rabbit Polyclonal

catalog :

MBS766304

quantity :

0.1 mg

price :

265 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with MBS766304 (ACAT1 antibody) at dilution of 1:1000

image 2 :

Immunohistochemistry of paraffin-embedded human liver using MBS766304 (ACAT1 antibody) at dilution of 1:100

product information

catalog number :

MBS766304

products type :

Antibody

products full name :

ACAT1 Rabbit Polyclonal

products short name :

[ACAT1]

products name syn :

[ACAT, ACAT1, Acetoacetyl CoA thiolase, MAT, T2, THIL]

other names :

[acetyl-CoA acetyltransferase, mitochondrial; Acetyl-CoA acetyltransferase, mitochondrial; acetyl-CoA acetyltransferase, mitochondrial; acetyl-CoA acetyltransferase 1; Acetoacetyl-CoA thiolase; T2]

products gene name :

[ACAT1]

other gene names :

[ACAT1; ACAT1; T2; MAT; ACAT; THIL; ACAT; MAT]

uniprot entry name :

THIL_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse; other species are not tested

sequence length :

427

specificity :

Please decide the specificity by homology

purity :

>=95% as determined by SDS-PAGE. Immunogen Affinity Purified

form :

Liquid

storage stability :

Store at -20° for 24 months (Avoid repeated freeze / thaw cycles.

tested application :

ELISA, WB, IHC

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Acetyl-Coenzyme A acetyltransferase 1. Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

other info2 :

Calculated MW: 40 kDa

products description :

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of cetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

ncbi gi num :

4557237

ncbi acc num :

NP_000010.1

ncbi gb acc num :

NM_000019.3

ncbi pathways :

Branched-chain Amino Acid Catabolism Pathway (1270161); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (413387); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (468288); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Fatty Acid Beta Oxidation Pathway (198865); Fatty Acid Degradation Pathway (82935); Fatty Acid Degradation Pathway (296)

ncbi summary :

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

uniprot summary :

ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family. Protein type: Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - tryptophan; EC 2.3.1.9; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Lipid Metabolism - fatty acid; Acetyltransferase; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - propanoate; Mitochondrial; Amino Acid Metabolism - lysine degradation; Lipid Metabolism - synthesis and degradation of ketone bodies. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion. Molecular Function: acetyl-CoA C-acetyltransferase activity; coenzyme binding; enzyme binding; metal ion binding; protein homodimerization activity. Biological Process: brain development; branched chain family amino acid catabolic process; ketone body biosynthetic process; ketone body catabolic process; liver development; protein homooligomerization; response to hormone stimulus; response to organic cyclic substance; response to starvation. Disease: Alpha-methylacetoacetic Aciduria

size1 :

0.1 mg

price1 :

265 USD

more info or order :

MyBioSource product webpage