catalog number :
MBS766199
products type :
ELISA Kit
products full name :
Rat T (Testosterone)
products short name :
[T (Testosterone)]
specificity :
This assay has high sensitivity and excellent specificity for detection of Biotin. No significant cross-reactivity or interference between Biotin and analogues is observed.
storage stability :
4 degree C for 6 months
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Competitive. Detection Range: 25-1600ng/ml. Sensitivity: <15ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Biotin were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Biotin were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with SA. During the reaction, Biotin in the sample or standard competes with a fixed amount of HRP-biotin. Excess conjugate and unbound sample or standard are washed from the plate. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Biotin in the samples is then determined by comparing the O D of the samples to the standard curve.
size4 :
10x96-Strip-Wells