This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human GGH (Gamma-glutamyl hydrolase)
catalog :
MBS766145
quantity :
48-Strip-Wells
price :
300 USD
product information
catalog number :
MBS766145
products type :
ELISA Kit
products full name :
Human GGH (Gamma-glutamyl hydrolase)
products short name :
[GGH (Gamma-glutamyl hydrolase)]
other names :
[gamma-glutamyl hydrolase; Gamma-glutamyl hydrolase; gamma-glutamyl hydrolase; gamma-glutamyl hydrolase; Conjugase; GH; Gamma-Glu-X carboxypeptidase]
products gene name :
[GGH]
other gene names :
[GGH; GGH; GH]
uniprot entry name :
GGH_HUMAN
reactivity :
Human
sequence length :
318
specificity :
This assay has high sensitivity and excellent specificity for detection of GGH. No significant cross-reactivity or interference between GGH and analogues was observed.
storage stability :
4 degree C for 6 months
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 0.469-30ng/ml. Sensitivity: 0.281ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GGH were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GGH were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GGH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GGH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GGH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GGH can be calculated.
ncbi gi num :
4503987
ncbi acc num :
NP_003869.1
ncbi gb acc num :
NM_003878.2
ncbi mol weight :
35,964 Da
ncbi pathways :
Fluoropyrimidine Activity Pathway (198778); Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Glutamate Removal From Folates Pathway (142235); Glutathione-mediated Detoxification Pathway (142435)
ncbi summary :
This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]
uniprot summary :
GGH: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Belongs to the peptidase C26 family. Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; Secreted, signal peptide; EC 3.4.19.9; Protease; Secreted. Chromosomal Location of Human Ortholog: 8q12.3. Cellular Component: cytosol; extracellular space; lysosome; melanosome; nucleus; vacuole. Molecular Function: exopeptidase activity; omega peptidase activity. Biological Process: glutamine metabolic process; proteolysis; response to drug; response to ethanol; response to insulin stimulus; response to zinc ion; tetrahydrofolylpolyglutamate metabolic process
size1 :
48-Strip-Wells
price1 :
300 USD
size2 :
96-Strip-Wells
price2 :
415
size3 :
5x96-Strip-Wells
price3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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